The Research Of Sang Xing Lung-protection Particles To Reduce Overexpression Of Th2 Cells In The Radiation Pulmonary Fibrosis

ObjectiveTo research the effect of Sang Xing Lung-Protection Particles（SX）on the overexpression of Th2 cells in rats with X-ray induced in pulmonary fibrosis.To clarify the mechanism of action of SX on the Radiation Pulmonary Fibrosis（RPF）.Method192 male SD rats,8weeks old and average weight 200±20g were randomly divided into blank group（given normal saline 10ml/d）,model group（given normal saline 10ml/d）,hormone group（given prednisone tablets aqueous solution with the concentration of 0.2mg/ml,15.60ml/Kg,10ml/d）,Sang Xing Lung-Protection Particles groups（given SX concentrate with the concentration of 2g/ml,5.6ml/Kg,11.25ml/Kg,22.50ml/Kg,10ml/d）,except for the blank group,the other groups were given X ray chest single irradiation 14Gy,all the animals were given with gavage once a day.At designated time(12^th,16^th,20^th,24^thweek)8 rats from each group were randomly executed,acquired lung tissue.The pathological changes of lung tissue was observed by Masson staining,the Real time-Polymerase Chain Reaction was used to detected the mRNA of IL-4,IL-5 and IL-13 in lung tissue.Results1.Pulmonary fibrosis process:no fibrosis were found in lung tissue of the blank group rats;at12^thh week each group rats that were irradiated still mainly showing inflammatio,at16^thh week after irradiation,each group rats began to present the pulmonary fibrosis,at 24^thh week end of the experiment mainly presented the pulmonary fibrosis.2.The expression of the blank group IL-4mRNA,IL-5m RNA and IL-13mRNA has been at a low level;compared with the blank group,the expression levels of each irradiation group IL-4mRNA,IL-5mRNA and IL-13mRNA increased to varying degrees（p<0.05）;compared with the model group,the expression level of the hormone group and SX groups IL-4 mRNA,IL-5 mRNA and IL-13 mRNA at different points in time decreased（p<0.05）;compared with the hormone group,at12^th,16^thh weeks,the expression of SX groups IL-4 mRNA,IL-5 mRNA and IL-13mRNA increased（p<0.05）,and at20^th,24^thh weeks,the expression decreased;there was no difference between the SX groups（p>0.05）.Conclusion1.14Gy irradiation agent could successfully establish the rats model of radiation pulmonary fibrosis,at16^thh week after irradiation,each group rats all presented different degrees of pulmonary fibrosis.3.In the process of the RPF,SX can reduce the mRNA expression levels of Th2related cytokines IL-4,IL-5,IL-13 in lung tissue.4.The effect of prednisone is significantly better than the SX in early of RPF,this may be related to its strong and rapid anti-inflammatory and immunosuppressive effects.