Sevoflurane Postconditioning Cardioprotection In Diabetic Rats Model Of Myocardial Ischemia Reperfusion: The Relationship With Mitochondrial Fusion Protein 1

Objective: To evaluate the change of myocardial mitochondria fusion protein in the state of diabetes,and diabetic rats myocardial with reperfusion processing and sevoflurane post-conditioning respectively,detection of myocardial mitochondria Mfn1 expression,discusses diabetes rat myocardial mitochondria Mfn1 expression in reperfusion processing and sevoflurane post-conditioning of change,reveals the effects of sevoflurane post-conditioning in diabetic rats myocardial reperfusion injury.Method: Sixty clean healthy adult Sprague-Dawley rats,weight 200?240g,using a random number table method,it is divided into healthy control group(S group),control diabetes group(DS group),ischemia-reperfusion group with the diabetes mellitus(DI/R group)and sevoflurane postconditioning diabetic group(DSev group).The preparation of diabetic rats model using disposable large(65 mg/kg)chain of urea with streptozotocin in abdominal cavity injection method.Myocardial ischemia-reperfusion injury model by left anterior descending coronary artery(LAD)ligation 30 min,loose line 120 min reperfusion method.S and DS group only for threading,DI/R group and DSev group ligation LAD complete ischemia reperfusion,which the sevoflurane(2.4 vol %)was inhaled for 5 minutes starts from 29 min of ischemia in DSev group.At the end of reperfusion,rats were sacrificed and cardiac tissues were obtained.The myocardial infarction size was measured by TTC staining method.The Myocardial Casepase3 expression level used Western blot method.The expression of myocardial tissue mitochondrial Mfn1 protein levels was determined by immune histochemical method.Result: Group of myocardial infarction compared with S group,DS group range change wsa not insignificant(P>0.05),Casepase3 expression was increased,the expression of myocardial mitochondria Mfn1 reduced(P<0.05),DI/R and DSev group increased myocardial infarction range,Casepase3 expression was increased,the expression of myocardial mitochondria Mfn1 reduced(P<0.05).Compared with the DI/R group,DSev group of myocardial infaraction area has no meaning in the study of statistical difference(P>0.05).Conclusion: The expression of diabetic rats myocardial Mfn1 than normal rats decreased significantly,diabetic rats myocardial reperfusion injury can futher reduce the expression of mitochondria Mfnl.Sevoflurane postconditioning does not apparent affect the expression of Mfn1 in diabetes rats which was going with reperfusion injury.