The Effect Of Pseudoprodioscin On The Lps-induced Expressions Of Inflammatory Mediators And The Relative Pathway In Raw264.7 Macrophage Cells

Objective:To explore the effects of pseudoprotodioscin on lipopolysaccharide-induced inflammatory expressions and the mechanisms resonsible for its anti-inflammatory effects in Raw 264.7 cells.Method:?1?Using High performance liquid chromatography to control the purity of experimental drug;?2?Raw264.7 cells were exposured with different concentration pseudoprotodioscin,using the Sulforhodamine B methods to detect the effect of pseudoprotodioscin on the proliferation of Raw264.7 cells;?3?Raw264.7 cells were treated with PPD and exposured with lipopolysaccharide,using qPCR and western blots to detect the expression of IL-1?mRNA?IL-1?protein,the relative gene and the protein expressions in the TLR2/MAPK/NF-?B/NLRP3 signaling pathway were also detected by qPCR and western blots methods;?4?Raw 264.7 cells were treated with PPD and exposured with lipopolysaccharide,applying qPCR?ELISA methods and western blot to detect the expressions of the relative gene and the protein in the arachidonic acid pathway.Result:?1?The purity of PPD was 96.15%,which could meet the requierement;?2?PPD?15-30?g/mL?has no effect on the the proliferation of Raw 264.7 cells;?3?The qRT-PCR and western blot showed that PPD inhibites the expressions of IL-1?mRNA?IL-1?protein and the activation of MAPK/NF-?B signaling pathway,PPD also inhibites the expressions of NLRP3/caspase-1 pathway;?4?PPD inhibites the expressions of the relative gene and the protein in the arachidonic acid pathway.Conclusion:Pseudoprotodioscin has significant anti-inflammatory activity by inhibite the expression of IL-1??LTB₄ and PGE₂,the anti-inflammatory mechanism of PPD is associated with p38MAPK/NF-?B?NLRP3/caspase-1 signaling pathway and arachidonic acid pathway.