| Objective:To investigate whether angiotensin(1-7)[Ang-(1-7)] can improve pancreatic islet morphology and function by up-regulating GLUT-2.Methods:Wistar rats,male,normal,8-week-old,34,adaptive feeding for 2 weeks,randomly selected 10 normal control group(NC,n = 10).All remaining diabetics were modeled,fed with high-calorie diet for 8 weeks,induced by streptozotocin,and20 models survived.They were randomly divided into two groups: the diabetic control group and the Ang-(1-7)intervention group.In the Ang-(1-7)intervention group,the right amount of Ang-(1-7)was subcutaneously injected into the neck of the experimental animal,and the other two groups were given the same volume of normal saline.Post-HE method was used to stain the islets of rats in each group and analysed by microscopy.Blood glucose,insulin and AngII levels were measured in each group,insulin resistance index(Homa-IR)was evaluated,and pancreas was detected by Western blotting.GLUT-2 protein expression level.Result:Compared with the normal control group,the weight of diabetic rats significantly decreased,serum glucose concentration,serum Ang II content and insulin resistance index increased,serum insulin levels decreased significantly,the difference was statistically significant(P <0.05);rat islets The structure of GLUT-2 protein in pancreatic islets was significantly reduced(P<0.05).Compared with diabetic group,the weight of rats in Ang-(1-7)intervention group was basically unchanged(P>0.05),serum glucose,serum AngII and Homa-IR were all decreased(P<0.05),serum insulinwas increased(P <0.05);Rat pancreatic islet contour and structure have improved,but can not reach the level of normal control group;GLUT-2 protein expression in islets increased(P <0.05).Conclusions:The results of the present study show that Ang-(1-7)treatment might decrease the level of blood glucose,suggesting an improvement of insulin resistance,which might associate with the upregulation of the PI3K/Akt signaling pathway in type 2 diabetic rats. |
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