Magnetic Polymer Microsphere Technology And Its Evaluation In Removing Endotoxin

Aptamers as a kind of oligonucleotide sequence with high affinity and specificity,good stability,low immunogenicity,which has in targeting drugs,biosensors,drug isolated clinical diagnosis broad application prospects.EAQ3 and B2 two sequences shows a sequence-specific binding affinity to the endotoxin,get to remove endotoxins from mixed solution.Amplified by PCR technology EAQ3 and B2 sequences obtained sequences amplified construct aptamers-beads and aptamer system-membrane system.The affinity of the two sequences were compared and then use them to remove endotoxins.Analyze the secondary structure of two sequences by DNAMAN 6.0.3.99,it showed that the two sequences are formed stem-loop structure,and the cyclic structure formed by the same number.After PCR amplification system and conditions were ssDNA EAQ3 explore and B2 sequence.The use of amplified ssDNA aptamers prepared-Bead system,after adsorption kinetics curve fitting,EAQ3 and B2 internal sequences within 10 minutes,respectively,the amount of endotoxin adsorbed 1207 EU/g and 991 EU/g,the adsorption good effect,the process quickly.Aptamers-within the membrane separation system toxin experiments,the highest adsorption capacity EAQ3 and B2 sequences were also reached the 1063 EU/mL and 954 EU/mL.Experimental results show that,compared B2 sequence,EAQ3 sequence isolated applications including toxins performance better affinity may be applied to the separation of biological products within the toxins.