Distribution And Changes Of Connexin 43 And Connexin 45 In Human With Chronic Calculous Cholecystitis

Background:The gallbladder is an important part of the biliary system.Gallbladder through absorption,secretion and movement play a concentration,storage and discharge of physiological functions.Gallbladder or bile duct dynamic start of organs.Many scholars have studied the anatomy and physiology of the gallbladder and its dysfunction can cause a number of diseases such as pancreatitis,cholecystitis,gallstone formation and sphincter of Oddi dysfunction.Chronic calculous cholecystitis is clinically more common diseases of the biliary system are physics,chemistry,neural and humoral factors such as result of which power weakened in the pathogenesis of biliary tract plays an important role in,but research has yet been fully clarify the mechanism of biliary Dynamics change.Current mechanism already provides a more in-depth study of gastrointestinal motility,?Interstitial cells of Cajal distributed the kind of pacing and gastrointestinal function and neurotransmitter functions of specialized cells,many gastrointestinal dynamic diseases has its direct or indirect participation,such as diabetic gastroparesis and Hirschsprung's disease.The ICC in the gastrointestinal smooth muscle with the surrounding wide gap junction between smooth muscle cells,and the network structure play a key role in the gastrointestinal dynamic mechanism.Biliary tract and gastrointestinal tract walls have similar structures.In people with chronic calculous cholecystitis in the process of dynamic mechanism change,study of the distribution of the ICC have been reported,but at the molecular level research ICC specific protein C-whether the expression of kit anomaly and the relationship with biliary power changes,and the expression of Cx43 and Cx45 and distribution and its relationship with biliary dynamics,the reports have not been at home and abroad.Objective:Discusses the distribution and expression of Cx43 ? Cx45 and ICC in biliary of chronic calculous cholecystitis,and the ICC,Cx43 and Cx45 relations with biliary dynamics.Methods: 1.Collection of surgical resection of chronic calculous cholecystitis in cholecystitis and non-non-inflammatory polyps of the gallbladder the gallbladder tumor specimens as the experimental group and the control group.HE stained inflammation observed specimens to determine whether the specimens into groups.Immunohistochemical technique to detect the expression of Cx43 and Cx45 gallbladder,its expression should be yellowish-brown granules,observe and do statistical analysis.2.Using Western blot techniques to the gallbladder C-kit,Cx43 and Cx45 testing and statistical analysis.3.Using RT-PCR to detect the Cystic C-kit,Cx43 and Cx45 m RNA expression and do statistical analysis.4.The gallbladder immunofluorescence double staining,Mark C-kit and Cx43 and Cx45 and C-kit,respectively,and the use of laser scanning confocal microscope and testing the distribution and expression of gap junction proteins Cx43 and Cx45.Results: 1.Compared with the control group in gallbladder,chronic calculous cholecystitis and gallbladder in Brown the sparse distribution of particles,and to reduce their number.2.The results of western blot showes that Chronic calculous cholecystitis and gallbladder C-kit,expression of Cx43 and Cx45 significantly decreased(p<0.01).3.The results of RT-PCR showes that Chronic calculous cholecystitis and gallbladder C-kit,expression of Cx43 and Cx43 m RNA decreased significantly(p<0.01).4.Confocal laser scanning microscopy shows: Interstitial cells of Cajal in the control group clearly was scattered in distribution,issued both ends of the cell processes?The expression of C-kit?Cx43 and Cx45 are increased?Positive expression of Cx43 with C-kit has a large area of the overlapping area.Conclusion: In human with Chronic calculus cholecystitis,expression of C-kit,Cx43 and Cx45 weakened and reduced distribution,suggesting chronic calculous cholecystitis of gallbladder reduce destruction of ICC.The gap junction of ICC to gallbladder smooth muscle cells,gap junction between smooth muscle cells,gap junction between ICC reduced,this may be an important factor leading to its gallbladder contractility decreased.