| Objective:To explore the association between single nucleotide polymorphisms of the gene of Nexilin and morbility of type 2 diabetes mellitus(T2DM)in Han Chinese of the Yan Bian Area.Methods:1.In Han Chinese of the Yan Bian Area,by a case-control design,selected 372 cases of type 2 diabetes as well as their age,sex-matched non-diabetic control group of 366 cases as research subjects,using TaqMan MGB probe to detect the subjects Nexilin gene rs 1166698 locus and allele,and tested the type 2 diabetes group and control group genotype and allele frequency distribution of the men and women,with type 2 diabetes group and control group,genotype and allele distribution.2.Detected of the targets height,weight and blood pressure,serum TC,TG,HDL,LDL,Glu,Urea,Cr,UA level,with automatic biochemical analyzer,and Hct through routine blood analyzer.The last,analyzed the Nexilin gene rs1166698 site genotypes and allele frequency distribution of the above indicators and the relationship of the distribution and the susceptibility for T2DM.Results:1.In Han Chinese of the Yan Bian Area,rs1166698 locus and allele frequencies in T2DM group and control group were in the law of Hardy-Weinberg equilibrium(P>0.05),and the sexuality between the groups was no significant difference(P>0.05).2.The results of the analysis were:genotype frequency was AA14.8%,GA46.5%,GG38.7%in the group T2DM and it was GG30.9%,GA47.5%,AA21.6%in the control group.rs1166698 locus GG genotype frequencies in T2DM group was significantly higher(38.7%>30.9%,P=0.018),and AA genotype frequencies in T2DM group was obviously lower than in the control(14.8%<21.6%,P=0.018).Allele frequencies between the two groups were also very significant differences,it showed G allele frequency in T2DM was significantly higher(62.0%>54.6%,P=0.004)and A allele in T2DM obviously lower(38.0%<45.4%,P=0.004)in Han Chinese of the Yan Bian Area.3.We collected a total of 315 female cases,including 158 T2DM patients and 157 people in the control group.The T2DM group genotype frequencies were GG%44.3,GA%42.4,AA%13.3,while the control group which genotype frequencies GG31.9%,GA47.1%,AA21.0%.GG genotype rs1166698 locus in the T2DM group frequency was significantly higher(44.3%>31.9%,P=0.042)and the AA genotype was obviously lower than in the control(13.3%<21.0%,P=0.042).The T2DM group allele frequency were G%65.5,A%34.5 and in control group were G%55.4,A%44.6.So in the T2DM,G allele frequency was significantly higher(65.5%>34.5%,P=0.011)and A allele in T2DM significantly lower(13.3%<21.0%,P=0.011).Interestingly,that differences did not exist in the groupe of the male in Han Chinese of the Yan Bian Area.4.In the recessive model(AA GA+GG),we were used to analyze genotype(GG+GA)sample in the T2DM prevalence increased significantly compared with the control group(85.48%>78.45%,P=0.01),while the dominant model no significant difference in the two groups in Han Chinese of the Yan Bian Area.5.The SBP,Urea,UA and Cr were increased in rs1166698 risk genotype GG samples(P<0.01).Conclusions1.Nexilin gene rs1166698 single nucleotide polymorphisms(SNPs)showed marginally significant associations with a higher risk of T2DM.rs1166698 locus GG genotype was positively correlated with T2DM susceptibility,and AA genotype was positively correlated with T2DM susceptibility.On the other hand,the allele G was the T2DM risk gene,and the allele A may be a protective factor in Han Chinese of the Yan Bian Area.2.In Han Chinese of the Yan Bian Area,rs1166698 single nucleotide polymorphisms(SNPs)associated with T2DM susceptibility to gender differences.In the female samples rs1166698 GG genotype was a positive correlation with T2DM susceptibility,the allele G was a T2DM risk gene,while in the male samples was not significantly different.3.In Han Chinese of the Yan Bian Area,rs1166698 locus showed recessive genetic model and the allele G was a T2DM risk gene.4.The risk factor G can increase four clinical indexes,including SBP、Urea、Cr and UA. |
