The Study Of Therapeutic Effect On Acute Ischemic Stroke Complicated With Hyperlipidemia By Buyang Huanwu Decoction Compound Granules

PurposeThe purpose of this study is to explore the therapeutic effect on acute ischemic stroke complicated with hyperlipidemia by Buyang Huanwu Decoction Compound Granules(BYHWDCG)?Frist of all,SD mice were selected to estabilsh the disease model of acute ischemic stroke complicated with hyperlipidemia.After a 3 weeks consecutive intragastric administration,to probe the therapeutic mechanism of BYHWDCG by detecting the whole blood cell analysis,haematological index including blood viscosity,plasma viscosity and Casson Viscosity,four coagulation tests,TC,TG,LDL_C,HDL_C and observing the ethology changes about model rats.Methods(1)Establish the hyperlipidemia animal modelSD male rats were selected to establish the hyperlipidemia animal model.2%cholesterol,0.5%sodium cholate,10%lard,5%yolk powder and 82.5%fundamental feed were smashed and mixed to fabricate the high-fat diet.After a 30 days consecutive feed with 100 g/kg weight of rats,detecting the levels of TC,TG,LDL C and HDL_C to evaluate the blood conditions of model rats.The livers of rats were dissected and weigh to explore the influence of model rats.Meanwhile,the other SD male were fed with normal diet as control group.Same measure methods were used to compare with model group.(2)Establish the acute ischemic stroke complicated with hyperlipidemia animal model.Modified Pulsinelli four-vessel occlusion(4-vo)operation was used on the hyperlipidemia animal to establish the acute ischemic stroke complicated with hyperlipidemia animal model.Evaluation methods designed by Wu were selected to evaluate the animal weather suit for the research,all the failed animals removed from the model group.After the operation.the hippocampus of rats' brains and livers were dissected to run the pathologic tests.Also,we detected the levels of the whole blood cell analysis,haematological index including blood viscosity,plasma viscosity and Casson viscosity,four coagulation tests to evaluate the blood condition of acute ischemic stroke complicated with hyperlipidemia animal.At the same time,sham operation were done on 8 normal rats,only incised the back between two ears and the neck of rats.All the sham operation rats will divide into sham group.Finally,8 totally normal rats will divide into control group.Same measurements of model group were used on sham and control group.All the results analyzed together,in order to compare the influences about 4-vo operation and access the consequences the model creation.(3)The therapeutic effects on the acute ischemic stroke complicated with hyperlipidemia rats of BYHWDCG.All the successful model rats divided into 4 groups,model group,BYHWDCG high dosage(30.4 g/kg),BYHWDCG medium doasge(15.2g/kg)and positive control group(0.4 g/kg,Naoxintong).8 sham operation rats divided into sham group and 8 normal rats divided into control group.3 weeks consecutive intragastric administration to all groups of rats of corresponding drugs or distilled water,and all of them were fed will normal diet.In the end of the research,all groups of rats executed and dissected the brains including the hippocampus and livers and stored in formaldehyde-phosphate buffer solution.All needs of blood drained from aortaventralis of rats and stored into blood collection tubes with different types of anticoagulation or plain tube.The whole blood cell analysis,haematological index including blood viscosity,plasma viscosity and Casson Viscosity,four coagulation tests,TC,TG,LDL_C,HDL_C analyzed together to access the whole blood condition.Livers of rats weighed for the size evaluation along with brain tissues to run the pathological tests to verify the changes after the treatment.Results(1)Establish the hyperlipidemia animal modelModel groups of hyperlipidemia rats shows a significant increase of TC,TG and LDL_C,and decrease of HDL_C compare with the control group,which matched the hyperlipidemia blood fat condition of human.Pathologic tests of livers show that mild steatosis was detected in all the model rats' liver cells.(2)Establish the acute ischemic stroke complicated with hyperlipidemia animal model.Acute ischemic stroke complicated with hyperlipidemia model rats show a sharp increase of WBC,NEU,PLT,PCT,HGB and RBC while compare with the sham and control group(P<0.05).Also a significant increase was observed in whole blood viscosity,plasma viscosity and Casson viscosity along with APTT are great shorter than control group and the levels of FIB_C are higher than control group(P<0.05).In pathologic tests,remarkable nerve cell pyknosis were found.About ethology changes,hemiplegia or myasthenia of contralateral limbs and other nerve cell trauma signs were observed in model group along with low spirits;eye balls turn black,thinning hair and decrease of locomotor activities.(3)The therapeutic effects on the acute ischemic stroke complicated with hyperlipidemia rats of BYHWDCG.Two groups of BYHWDCG can lower the abnormal level of WBC,NEU,PLT,PCT,RBC and HGB in rats whole blood analysis.In whole blood viscosity,plasma viscosity and Casson viscosity,two groups of BYHWDCG show a significant down regulation of the abnormal blood condition.Regretfully,BYHWDCG cannot longer the APTT but can lower the levels of FIB_C successfully.In blood fat tests,BYHWDCG can lower the levels the TC,TG,LDL C.We could also noticed that BYHWDCG can narrow the unusual size of model rats.In pathologic tests,mild steatosis of liver cells condition were improved and approached the normal rats after BYHWDCG treatment and nerve cell pyknosis condition improved too.In ethology changes,two groups of BYHWDCG can enhance the capacity of motion of acute ischemic stroke complicated with hyperlipidemia rats,inspire spirits and remitted the nerve trauma sign.ConclusionThe methods we used to established the acute ischemic stroke complicated with hyperlipidemia animal model is solid credible,all the model rats signs match with clinical acute ischemic stroke complicated with hyperlipidemia patients.And BYHWDCG shows a great help in treatment with acute ischemic stroke complicated with hyperlipidemia rats.So,our research can provide a new idea of traditional Chinese medicines' twice sharping and provide a reliable treatment about how to cure acute ischemic stroke complicated with hyperlipidemia and offer laboratory data for clinical use.