Study On The Processing Technology And The Quality Of Ling Nan Pao Tianxiong

ObjectiveStudy on the processing technology of Lingnan Pao Tianxiong,find out and optimize single factors.Then the orthogonal test was designed to examine the processing technology of Lingnan Pao Tianxiong.We used the Lingnan Pao Tianxiong decoction with administrate by gavage to observe the acute toxicity of the mice in this study and proof the security in it.To find out and analyze the current using situation in clinical by distributing the questionnaires which will provide a reference for further safety evaluation of Lingnan Pao Tianxiong.Study on the quality of Lingnan Pao Tianxiong,and analyze the different qualities of Lingnan Pao Tianxiong,Pao Fupian and Sichuan Pao Tianxiong.Finally the quality standard of Lingnan Pao Tianxiong would be drafted by the experimental results,which are benefit for us to discover the significance of Lingnan Traditional decoction pieces.Methods1 a study on the processing technologyWe used the limit content of diester-type alkaloids and the content of monoester-type alkaloid as index,used single factor test as soaking time,soaking with different proportions of ginger,steaming time,fried temperature with sand.And the orthogonal test was designed to examine the processing technology of Lingnan Pao Tianxiong.2 a study of acute toxicity in miceWe used administrate the Lingnan Pao Tianxiong decoction by gavage to observe the acute toxicity of the mice in this study,and explored the maximum dose of Lingnan Pao Tianxiong.We analyzed the different acute toxicity of Lingnan Pao Tianxiong,Pao Fupian and Sichuan Pao Tianxiong.It will be better evaluate the security of Lingnan Pao Tianxiong,and will be protecting the drug safety in clinical.3 clinical investigationsDistributing the questionnaires to the doctors which are using Lingnan Pao Tianxiong in clinical,to find out the current using situation in clinical.It will provide a reference for further safety evaluation of Lingnan Pao Tianxiong.4 a study of quality standardPefer to 2010 edition“Chinese pharmacopoeia”,we determined the identification in appearances,microscopy,TCL,the moisture content,the total ash,the acid-insoluble ash,the content of water-solute,the limit content of diester-type alkaloids,the content of monoester-type alkaloid of the Lingnan Pao Tianxiong,And analyze the different qualities of Lingnan Pao Tianxiong,Pao Fupian and Sichuan Pao Tianxiong.Finally the quality standard of Lingnan Pao Tianxiong will be draft by the experimental results.Results1 a study on the processing technologyStudy on the processing technology of Lingnan Pao Tianxiong,optimize three single factors which are the soaking time,steaming time,fried temperature with sand.And the orthogonal test was designed to examine the processing technology of Lingnan Pao Tianxiong.The results showed that the best processing technology of Lingnan Pao Tianxiong is A2B2C3,that it is soaking 5d,soaking with 8%proportions of ginger,steaming 1.5h with high pressure,fried temperature 210?230?.2 a study of acute toxicity in miceThe acute toxicity of Lingnan Pao Tianxiong decoction did not casuse the abnormalities and the death of the mice in this study.The maximum dose of Lingnan Pao Tianxiong is 177.6g/kg.It can be converted into an adult dose base on the body surface area which is the 91 times of the clinical maximum dosage.And its maximum dosing is higher than Pao Fupian.Thus,Lingnan Pao Tianxiong have high security in clinically.3 clinical investigationAccording to the survey,Both men and wowen patients can use the Lingnan Pao Tianxiong for year-round.The adults,especiallyt the prime and the elderly which the age is more than 30 years are the mian user groups.Usually it can be use to cure the deficiency of the spleen-yang and insufficiency of the kidney-yang syndrome.One third of the doctors will use more than 15g each dose,indicating its less toxic,high security.4 a study of quality standardTo measure 17 batches of Lingnan Pao Tianxiong by identification in appearances,analysis of microscopy and TCL,measure the moisture content,the total ash,the acid-insoluble ash,the content of water-solute,the limit content of diester-type alkaloids and the content of monoester-type alkaloid.And analyze the different qualities of LingnanPao Tianxiong,Pao Fupian and Sichuan Pao Tianxiong.Finally the quality standard of Lingnan Pao Tianxiong will be draft.Conclusion1 a study on the processing technologyTo optimize and standardize the processing technology indicators of the Lingnan Pao Tianxiong.It will be benefit for providing basic methods and foundation for the large sacle prodction while improve product quality and production efficiency.2 a study of acute toxicity in miceIn this study,the acute toxicity of Lingnan Pao Tianxiong decoction did not casuse the abnormalities and the death of the mice,And its maximum dosing is higher than Pao Fupian that can be converted into an adult dose base on the body surface area which is the 91 times of the clinical maximum dosage.Those can proof the high security of it.3 clinical investigationAccording to the survey,Lingnan Pao Tianxiong is effective and high security in clinically.4 a study of quality standardThe quality standard of the Lingnan Pao Tianxiong is draft as follow:it ought to conforms the demand of identification in appearances,TCL,the moisture content cannot exceed 13.0%,the total ash canno exceed 5.0%,the acid-insoluble ash cannot exceed 5.0%,the content of water-solute extract cannot lower than 15.0%,the limit content of diester-type alkaloids cannot exceed 0.003%,the content of monoester-type alkaloid cannot lower than 0.010%.