Persimmon Leaf Flavonoids Against Melanoma Pharmacodynamics Research And The Preparation Of The Whitening Gels

[Object]Choose persimmon leaf flavonoids as the research object,research the pharmacodynamics of cells in vitro and in vivo pharmacodynamics study,examine whether it has the role of melanoma;The persimmon leaf flavonoids liposome preparation into gels,quality inspection of the liposome gels,inspects its exterior shape,particle size distribution,the encapsulation rate.[Method]Persimmon leaf flavonoid was extracted by ethanol reflux method.Choose the purification by polyamide column chromatography;Using ultraviolet spectrophotometry determination of total content of flavonoids in the persimmon leaf;Through the toxicity test of persimmon leaf flavonoids toxicity;Through the determined by MTT method to examine persimmon leaf flavonoids of melanoma A375 cells in vitro against melanoma pharmacodynamics experiment;With underarm injections model to melanoma B16 melanoma cells resist melanin pharmacodynamics experiment in tumors;Use ultrasonic dispersion of persimmon leaves flavonoids liposome prepared;By dextran gel column chromatography separation of free drugs,determination of coating rate;Coating rate as index,through the single factor investigation liposome and orthogonal test to determine the best prescription;Is given priority to with persimmon leaf flavonoids liposome drug,carbomer-940-C for gel matrix,propylene glycol and PEG-400 as moisturizer,triethanolamine as pH regulator,azone for transdermal promoter,preparation of gels;Through single factor and orthogonal test to determine the liposome gels best prescription.[Results]Persimmon leaf flavonoids extraction technology for three times 70%ethanol reflux,dosage of solvent was followed by 10 times,eight times,eight times,extracting time of 2 h,2 h,1 h,the resulting extracts of persimmon leaf flavonoids content is 45.5%;Preparation of persimmon leaf flavonoids liposome dosage of the best prescription for PC 100 mg,phospholipids and cholesterol ratio of 5:1,medicine fat than 1:8 measured using the best prescription of the preparation of liposomes,on average,the envelopment rate was 74.91%:The best technology of preparation of persimmon leaf flavonoids liposome gels for carbomer-940-C dosage was 1%,the moisturizer to choose PEG-4000 and share,propylene glycol,PEG-4000 was 10%,the dosage of propylene glycol addition amount of 10%,triethanolamine to adjust pH 5,azone addition amount of 0.005%.[Conclusion]Persimmon leaf extract with 75%ethanol reflux method to get high purity of flavonoids;Through the toxicity test found that persimmon leaf flavonoids on basic non-toxic ICR mice;Through the in vivo and in vitro pharmacodynamics experiment showed that persimmon leaves flavonoids－without the role of melanoma;Using thin-film ultrasonic dispersion of persimmon leaves flavonoids liposome prepared,its coating rate is high,narrow particle size distribution,uniform appearance;Preparation of persimmon leaf flavonoids liposome gels look fine,uniform and transparent,has certain slow-release characteristics of good stability.