The Research Of The BP₂₅₆ Protective Effect On Toxicity Of Cisplatin Damage

BP256 is a kind ofpeptide derived from food protein,with the process of enzymolysis.filtration and drying.Cis-platinum is a kind of drug that is nonspecific to cells,which is widely used cancer chemotherapy.And cis-platinum could cause injury to normal tissue cells while it makes effects on antitumor cells.Renal toxicity is a major side effects of cis-platinum,which greatly limits its clinical application.It is significant to find a kind of substance which could reduce the renal toxicity of cis-platinum and not abate its antitumor effect at the same time.1.The research of peptide derived from food protein BP256 was studied on the protective effecton renal toxicity caused by cis-platinum in mice.Cis-platinum injury animal model was set up by the injection of cis-platinum 20mg/kg b.w.one time.The experiment groups were fed with BP256 in different concentrations for 7 consecutive days after 3 days and 3 days before the cis-platinum injection in mice.Kidney tissue pathological slice showed that cis-platinum model group had obvious inflammatory cell infiltration.malpighian tube skin cell fall off,and severely necrotized.The group fed with BP256 did not see obvious inflammatory cell infiltration or cells necrosis.In the cis-platinum model group,it has been found that the content of the serum urea nitrogen(BUN)and the creatinine(Scr)rise.the MDA content increased in the kidney tissues and the activity of SOD and GSH-PX decreased.The result showed that the content of the BUN and the Scr decreased in the group fed with BP256.What's more,there is a better effect in the middle dose group than the low-dose group and high-dose group.And the content of MDA in BP256 group dropped to a varying degree.The activity of the SOD increased compared with the cis-platinum model group.There was a significant difference between the CDDP model group and the medium and high dose group.And it has been found that the activity of the GSH-PX improved greatly in kidney tissue with the BP256 low dose group,and there was significant difference contrast with the CDDP model group.It has been demonstrated that food-borne peptide BP256 has protective effect on renal toxicity caused by cis-platinum in mice.2.The research of peptide derived from food protein BP256 was studied on the protective effect on cell injury caused by cis-platinum in HUVEC-12cell.The effect on the activity of the HUVEC-12 cell proliferation was observed when the HUVEC-12 cell was cultured with BP256.The optimal concentration and the function time have been screened of the inhibition ratio on HUVEC-12 with the cis-platinum content of 0.125?0.5?2.5?5?10?20 ?g/mL.And the contents of the SOD activity were been determinate in each cell culture supernatant.There was no obvious effect on HUVEC-12 cell proliferation of the BP256.The HUVEC-12 cell model injury has been established caused by cis-platinum.The concentrate was 2 ?g/mL and the function time was 48 hours.It has been found that the cell survival rate increased in cell group protected with BP256 compared with the cell model injury group caused by cis-platinum with the MTT method.Single cell gel electrophoresis results showed that there was a significant difference in cell percentage of tail length,tail moment,tail DNA content,olive tail moment when the cell model injury group compared with the normal group.It has been found BP256 could decrease the HUVEC-12 cell DNA damage.3.The influence of the BP256 on A549 cells has been investigated that was inhibited by cis-platinum.To study the influence of BP256 on A549,A549 cell has been cultured with BP256.The effect on A549 cell inhibition rate has been studied by observation of different initial inoculation cells,cis-platinum concentration and the action time.The A549 cells inhibited by cis-platinum have been studied by BP256 combined with different concentration cis-platinum.The A549 cells model inhibited by cis-platinum have been established with the initial inoculation cells 5�103and the cis-platinum for 48 hours that the concentrations were 0.125?0.25?0.5?1?2 pg/mL.The inhibition rate was higher than cis-platinum alone group in the same concentration,which was acted by BP256 combined with various concentrations cis-platinum.The BP256 could sensibilize A549 cells on the inhibition of the cis-platinum.