Experimental Study Of Fumai Decoction On Ischemic Arrhythmia Of Mycocardial Enzyme And Phosphorylation Signal Pathway Of Cx43 Ser262

Objective Studied the effect of Fumai decoction on ischemic arrhythmia of rats in myocardial enzyme aspartate aminotransferase(AST),creatine kinase isoenzyme(CK-MB),cardiac troponin I(cTnI),occurrence time,duration time,numbers of ischemic ventricular arrhythmia and Cx43 Ser262 phosphorylation signaling pathway that mediated by ERK.And exploreing the effect and mechanism of Fumai decoction on ischemic arrhythmia.Methods The experiment used clean male SD rats,which is randomly divided into Sham operation group,Model group,Metoprolol tartrate group,Zhigancao decoction group,high,medium and low dose groups of Fumai decoction.After the administration in 14 days,rat models of ischemic arrhythmia is producted by ligating the left anterior descending artery(except sham group).(1)Detected the contain of AST CK-MB,cTn I in the serum of rats(2)Observed the change of myocardial tissue ultrastructure using transmission electron microscope(3)Recorded occurrence time,duration time,numbers of ischemic arrhythmia(4)Detected expression of ERK and phosphorylation of Cx43 Ser262 by Western-blot.Results1.Influence on content of myocardial enzymes AST,CK-MB,cTnI:Compared with the the model group,the content of AST,CK-MB and cTnI can be significantly reduced by the administration of each dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Compared with the Zhigancao decoction group,the content of AST,CK-MB and cTnI can be significantly reduced by high dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Compared with the Zhigancao decoction group,the content of AST,CK-MB could be cut down by medium and low dose group of Fumai decoction(P<0.05).Compared with the Zhigancao decoction group,the content of cTnI could reduced by medium dose group of Fumai decoction(P<0.05).2.Ultrastructural observation on myocardial cells:It is clear that Fumai decoction could significantly reduce the degree of myocardial ischemia injury in rats,which could improve the myocardial cell ultrastructure.Model group:myocardial cell myofibrils display fracture,distortion,shorten.The arrangement of mitochondrions is disorder.The structure of mitochondrions is damaged.Sham group:Z line of myocardial cells is rule and sarcomere is clear.The arrangement of mitochondrions dispaly neat.Zhigancao decoction group:Myocardial fibers are irregularly arranged and show contraction band.Part of the sarcomere is crack.The mitochondrions are irregularly arranged and obviously damaged.The arrangement of myofibrils is basically rule and the structure of mitochondrions is relatively complete in high,medium,low dose group of Fumai decoction and metoprolol group.3.Effect on ischemic arrhythmia:Compared with the the model group,arrhythmia occurrence time could be obviously delayed by the administration each dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Arrhythmia duration time could be obviously shorten by the administration each dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Numbers of ischemic ventricular arrhythmia could be obviously reduced by the administration each dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Compared with the Zhigancao decoction group,arrhythmia occurrence time could be markedly delayed by the high dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Arrhythmia duration time could be significantly shorten by the high of Fumai decoction and metoprolol tartrate group(P<0.01).Numbers of ischemic ventricular arrhythmia could be significantly reduced by the high dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Compared with the Zhigancao decoction group,arrhythmia occurrence time could be delayed by the medium dose group of Fumai decoction(P<0.05).Arrhythmia duration time could be shorten by the medium group of Fumai decoction group(P<0.05).Numbers of ischemic ventricular arrhythmia could be reduced by the medium and low dose groups of Fumai decoction(P<0.05)4.Inhibited phosphorylation of Cx43 Ser262 that ERK mediated:Compared with the the model group,the expression of ERK/GAPDH and Cx43 pser262/GAPDH can be significantly reduced by each dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Compared with the Zhigancao decoction group,the expression of ERK/GAPDH and Cx43 pser262/GAPDH can be significantly reduced by the high dose group of Fumai decoction and metoprolol tartrate group(P<0.01).Compared with the Zhigancao decoction group,the expression of ERK/GAPDH and Cx43 pser262/GAPDH could reduced by medium dose group of Fumai decoction(P<0.05).Conclusion1.Fumai decoction had protective effect which can decrease the content of serum myocardial enzyme AST,CK-MB,cTn I and reduce the injury of myocardial cell ultrastructure in acute myocardial ischemia.2.Fumai decoction had another effect in ischemia arrhythmia which postponing the arrhythmia occurrence time,shorting duration time,and reducing numbers of arrhythmia.3.Fumai decoction had the protective effection of ischemic arrhythmia based on regulating failure of gap junctional intercellular communication.The mechanism may be that Fumai decoction regulating phosphorylation of Cx43 Ser262 that ERK mediated.4.The protective effect of Fumai decoction on ischemic arrhythmia may be related to reducing myocardial ischemic injury,protecting integrity of myocardial cell membrane,reducing the content of AST?CK-MB?cTn I,ensuring the integrity of myofibrils and mitochondria of myocardial cells,inhibiting phosphorylation of Cx43ser262 that mediated ERK.