| Diabetes risk to human health in the 21st century to become the third largest of chronic non-communicable diseases,with changing lifestyles and living standards,the incidence of diabetes increased year by year,our country may have become the most number of diabetes Prevalence country.The main Points of diabetes type 1 and type 2,in which more than 90%of type 2 diabetes.Now widely recognized that type 2 diabetes is a Physiological and Pathological mechanisms of islet ? cell dysfunction and insulin resistance.Apoptosis in Pancreatic ? islet ? resulted in an increase in the number of cells and decreased islet ? cell dysfunction in diabetes throughout the Progression occupy an important role,so the research on how to Prevent islet P cell apoptosis,repair damaged islet P cells and Promote insulin secretion become a key treatment of diabetes.Sericin color cocoon is concentrated together by soaking,decoction,filtered,,which contains a variety of water-soluble Protein biological activity when reeling and refining are discarded as waste,the Public has the cocoon to soak hypoglycemic Prescription,domestic external effects of diabetes research in the sericin still rare,so the research aspect of the treatment of diabetes sericin can explore new ways of treatment of diabetes,on the other hand can be fully effective use of resources,turning waste into treasure.In this study,high-sugar high-fat diet Plus low-dose streptozotocin(STZ)intraperitoneal injection of Type 2 diabetic rat model to explore the effects of sericin on glucose and lipid metabolism and insulin in type 2 diabetic rats and morphology of Protective mechanisms of islet P cell injury,to Provide new ideas for the Prevention and treatment of diabetes and Provide a theoretical basis.Part 1 Influence on glucose and lipid metabolism and insulin in type 2 diabetic rat's morphologyObjective:The experiment by observing glue effect on glucose and lipid metabolism in type 2,insulin expression in pancreatic islet cells and islet morphology in diabetic rats,and to explore the mechanism of servicing Prevention of diabetes.Methods:1 Male,SD rats,weighing 200-250g,high-sugar high-fat diet(3%egg yolk,18%lard,20%sucrose and 59%diet)feeding 4 weeks,fasted 12 hours,for 3 days by intraperitoneal injection of STZ 25 mg/kg/d,continue to use the high-sugar high-fat diet for three weeks,disable high-sugar high-fat diet for 3 consecutive days was measured fasting blood glucose,blood glucose greater than 16.7mmol/L for the successful modeling.The successful modeling of stratified according to blood glucose levels in rats were randomly divided into diabetic model group(DM),metformin(MT),sericin treatment group(ST).Sericin treatment group sericin(2.4g/kg/d),rats given metformin metformin(55.33mg/kg/d)gavage times are 35 days;the normal control group(NC)and model group were not treated.2 Using the glucose oxidize test blood sugar(blood glucose,BG),oxidize colorimetric detection triglyceride(TG),total cholesterol(TC),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),copper reagent colorimetric detection of free fatty acids(free fatty acid,FFA).3 HE staining observed morphological changes in pancreatic islets.4 Islet cells were observed in the expression of insulin immunohistochemical staining method.Results:1 A blood biochemistry test resultsCompared with normal control group,BG model rats,TG,TC,LDL-C,FFA significantly increased,HDL-C was significantly lower,the difference was statistically significant(P<0.01,P<0.05).Compared with the model group,sericin treatment group,BG metformin rats,TG,TC,LDL-C,FFA significantly reduced,HDL-C was significantly increased,the difference was statistically significant(P<0.01).There was no significant difference(P>0.05)sericin treatment group and metformin group.2 Changes in islet morphologyNormal rat islets close evenly distributed,clean cut,the number of islet cells of normal;compared with the normal group,model group rat islets and uneven distribution of sparse,less rounded contours,edges irregular,reduce the number of islet cells;compared with the model group,the treatment group and the number of sericin metformin group increased islet cells were distributed more closely,outline more clearly.3 The expression in rat Pancreatic islet cells in each group of insulinLight microscopic structure of the normal control group intact rat islets,more full,clear boundaries,insulin-positive granules after staining brown,widely distributed in the islets;diabetic model group rat islets narrow,ill-defined,the expression levels of insulin decreased(P<0.05);rat islets sericin metformin group and the treatment group increases,the boundary more clearly elevated insulin expression compared with the model group(P<0.05),there is no obvious differences between those(P>0.05).Conclusion:1 Sericin hypoglycemic can lipid-lowering,weight loss,increased insulin sensitivity,improving the Pancreas Insulin resistance.2 Sericin can improve islet morphology damage,repair islet cells,Promote islet cell regeneration.3 Sericin can increase insulin synthesis,improve islet ?-cell function.Part 2 The protective effect of sericin diabetic rat Fas-mediated apoptosis in pancreatic ?Objective:In this study,the islet cells of type 2 diabetic rat interleukin-1?(IL-1?)and death receptor(Fas)expression concept glue explore sericin Prevent islet? cell apoptosis,repair islet P cells damage mechanism.Method:1 Male,SD rats,weighing 200-250g,high-sugar high-fat diet(3%egg yolk,18%lard,20%sucrose and 59%diet)feeding 4 weeks,fasted 12 hours,for 3 days by intraperitoneal injection of STZ 25 mg/kg/d,continue to use the high-sugar high-fat diet for three weeks,disable high-sugar high-fat diet for 3 consecutive days was measured fasting blood glucose,blood glucose greater than 16.7mmol/L for the successful modeling.The successful modeling of stratified according to blood glucose levels in rats were randomly divided into diabetic model group(DM),metformin(MT),sericin treatment group(ST).Sericin treatment group sericin(2.4g/kg/d),rats given metformin metformin(55.33mg/kg/d)gavage times are 35 days;the normal control group(NC)and model group were not treated.2 Immunohistochemical staining of islet cells in IL-1? and Fas expression.3 RT-PCR was used to detect the expression of islet cells of IL-1? and Fas.Results:1 Islet cells in rats expressing IL-1?The islet cells of diabetic rats group expressed IL-1? Protein and mRNA were higher than normal group(P<0.05);sericin and metformin treatment groups islet cells expressing IL-1? mRNA and Protein was significantly lower than diabetic model rats(P<0.05);and no significant difference sericin treatment group and metformin group(P>0.05).2 Islet cells in rats of Fas expressionThe expression in rat Pancreatic islets in diabetic model group Fas Protein and mRNA were higher than normal group(P<0.05);sericin treatment group and the islet cells in the metformin group Fas Protein and mRNA expression was significantly lower than that of diabetic model group(P<0.05);,and there was no significant difference between the treatment group and sericin metformin group(P>0.05).Conclusion:1 Sericin can reduce islet ? cell inflammation and improve the surrounding environment islet(3 cells.2 Sericin can reduce the expression of Fas death receptor,inhibition the Fas-mediated apoptosis. |
PDF Full Text Request