Studies On The Secondary Metabolites And Alpha-glucosidase Inhibitory Activity Of Inonotus Obliquus

Inonotus obliquus,a white rot fungus that belongs to the family Hymenochaetaceae of Basidiomycetes,is distributed in Europe,Asia,and North America.Traditionally,it is used in Russia,Poland and Japan for the treatment of gastrointestinal cancer,cardiovascular diseases and diabetes.Pharmacological investigations showed that Inonotus obliquus also possessed anti-inflammatory,antimutagenic,and hepatoprotective activities.To the good traditionally medicinal value,Inonotus obliquus attracts attention in new drug research and development.Based on the review on the chemical constituents and pharmacological research of Inonotus obliquus,the secondary metabolites were studied and their ?-glucosidase inhibitory activities were tested.Eighteen compounds were purified through chromatography separation(silica gel,RP-18,MCI and the preparative high performance liquid chromatography)from the secondary metabolites of Inonotus obliquus.On the basis of physicochemical properties and extensive spectroscopic analysis(IR,UV,MS,NMR,2D-NMR,CD and optical rotation),the chemical structures of these compounds were elucidated.Among the new structures(4,9,11,17,18),compounds 4 and 9 were identified as drimane sesquiterpenoids,11 was elucidated as alkaloid,while compounds 17 and 18 were identified as triterpenes.These three kinds of compounds were evaluated simply for their ?-glucosidase inhibitory activities.Triterpene compounds 17 and 18 have a good performance in restraining ?-glucosidase enzyme,as new chemical entities from Inonotus obliquus on treatment of diabetes.