| At present,hair follicles and skin are the hot spots in cell biology and skin wound repair and dermatology.It deals with the localization of hair follicle stem cells,morphological analysis of hair follicles,hair follicle signal transduction,growth factors,cytokines and dermis and epidermis The interaction between the various aspects of the biological function of the study.Hair follicle as an important structural and periodic regeneration of the skin in the important organs,the hair follicle stem cells plays an important role in the skin trauma repair and pathophysiological immune mechanism.To establish and improve the method of reconstructing new hair follicles in vitro and in vitro,and to explore the mechanism of hair follicle regeneration and the related mechanism can be used to further study the growth,development,differentiation and apoptosis of hair follicle cells.In addition,the conditions of fetal skin wound healing were optimized and the active region of the cells was established,which laid the foundation for the establishment of artificial skin minimally invasive model of fetal skin.At the same time,the preliminary analysis of the formation and migration direction of transit amplifying cells in newborn scars was provided,which provided theoretical support for the migration of stem cells in skin wound repair.1、Microscopic anatomical separation of hair follicles outer root sheath and in vitro culture.The results showed that the outer root sheath of the hair follicle could be reconstructed on the 5th day in vitro.The original fibrous membrane of the hair follicle was replaced by the newly formed pseudo-glass membrane as the wound in the process of hair follicle regeneration.Cell migration direction,to restore the new hair follicle regeneration process of three-dimensional spatial morphology.2 、 Optimization Medium for Mouse Fetal Skin Wound Healing Scarless and Analysis the Cells Proliferation Active Region.Results: After 3-day cultivation,wound healing rates significantly differed among the DMEM group,DMEM + 5% FBS group,DMEM + 10% FBS group,MEM group,MEM + 5%FBS group and MEM +10% FBS group(0,3.33%,6.67%,3.33%,46.67% and 26.67%,respectively,χ 2 =41.39,P < 0.05).Additionally,the MEM + 5% FBS group showed a significantly higher wound healing rate than the other groups except the MEM + 10% FBS group(all P < 0.01).Logistic regression analysis showed that the type of basal medium(MEM: OR = 11.717,95% CI: 3.274-41.934,P < 0.001)and FBS concentrations(5%FBS: OR = 24.625,95% CI: 3.027-200.299,P = 0.003;10% FBS: OR = 13.449,95%CI: 1.618-111.813,P = 0.016)were factors influencing fetal wound healing.Under the culture condition of5% FBS +MEM,the dermis and epidermis healed well without epidermal thickening.The cutaneous stem cell labeling technique showed that the papillary dermis and basal layer of the epidermis were the two major regions with active cell prolifera.3、The wound healing pattern of neonatal mice was observed.Results: The Sox2 positive cells in the epidermal layer cohered gradually during wound coalescence,and significant expressions of both Sox2 and CK14 were detected in the papillary layer of dermis.Meanwhile,in the initial stage of wound coalescence,cells stated migration primarily from the bottom of the wound surface,and moved upwards to fill in the wound.In summary,hair follicle outer root sheath in vitro culture to the first 5d can be the initial reconstruction of the basic form of hair follicles,hair follicle internal center can achieve scarless healing.MEM + 5% FBS culture condition is the optimum culture condition of fetal skin wound without scar healing,and the dermal papillary layer and epidermis basal layer of fetal skin are two major cell proliferation active regions in wound healing process.Initially,the initial migration of the wound began mainly from the bottom of the wound began to move up and fill the wound. |
