Application Of Nanoparticles Containing Docosahexaenoic Acid In Tumor Therapy

Cancer is a major disease caused by normal cell mutations in the body and has become the most important threat to human health today.At present,the main means of cancer treatment is still dependent on chemical therapy,but the vast majority of chemotherapeutic drugs are less specific,difficult to overcome the complex physiological barrier in the body,and susceptible to induce cancer multidrug resistance which eventually make chemotherapy failing.In order to target the tumor and reduce the side effects of drugs to overcome the multidrug resistance(MDR)and achieve the maximum effect of cancer treatment,it has become a great potential research direction to rely on nano-drug delivery system.Docosahexaenoic acid(DHA)is a vital member of the omega-3 polyunsaturated fatty acid family and has significant anti-inflammatory and anti-tumor effects.The aim of this study is to explore the effect of DHA nanoparticles in tumor therapy,and provide a theoretical reference for the development this new anticancer therapy.In this study,human hepatocellular carcinoma cell Be17402 and its drug resistant cell Be17402/Fu in vitro were detected with MTT experiments,cell uptake experiments,apoptosis detection experiments and western blot experiments,which were performed by NLC-DOX-DHA.These results showed that NLC-DOX-DHA could enhance the sensitivity of drug-resistant cells to anti-cancer drug doxorubicin and promote drug-resistant cell apoptosis.Then,NLC-DOX-DHA was injected into the nude mice model of subcutaneously inoculated with human hepatocellular carcinoma cell line Be17402/Fu.The body weight,tumor volume and weight of nude mice were measured and analyzed statistically.These results showed that NLC-DOX-DHA prolonged the circulating time of doxorubicin in vivo and enhanced the effect of doxorubicin on the prophylactic and cytotoxic effect of doxorubicin on solid tumors.At the same time,DHA flexible liposomes were prepared in this study.We simulate the vitro environment of doxorubicin's skin administration and percutaneous absorption in vivo.It was found that DHA flexible liposomes could effectively improve the transdermal absorption efficiency of doxorubicin.In conclusion,this study confirm that DHA contained nanoparticles can effectively improve the killing effect of anticancer drugs on tumors and improve the uptake efficiency of anticancer drugs towards cells and skin,suggesting that DHA contained nanoparticles may become a potential tumor chemotherapy aid.