The Effect Of Induced Pluripotent Stem Cells(iPSc)on The Growth And Metastasis Of Human Lung Cancer A549 Cells In Nude Mice

Human induced pluripotent stem cells(hiPS cells)are introduced into human somatic cells by gene-stable transfection techniques,reproduce somatic cells,which form pluripotent cells of pluripotent stem cell differentiation and Embryonic stem cell-like morphology.The pluripotency and differentiation potential of human iPS cells make it a useful tool for basic research.In this study,the effect of iPS cells on the growth and metastasis of human non-small cell lung cancer cell line A549 cells was studied by nude mice transplanted tumor model.OBJECTIVE:To establishment of nude mice iPS cells and A549 cells transplanted tumor model,observe the tumor formation and draw the tumor volume growth curve.The effects of iPS cells on the development of human non-small cell lung cancer(NSCLC)tumor cells were investigated by using the small animal live imaging technique to observe the development and metastasis of nude mice tumor model established in stable expression of fluorescent reporter gene cells.METHODS:BALB/c nude mice subcutaneous tumorigenesis method was used to inject different kinds of treated cells,and the phenotype and growth changes of A549 cells and iPS cells were observed The lung cancer animal model of transplanted A549 cells and iPS cells was established by using the gene stably transfected with fluorescent protein.The imaging effect of tumor bearing nude mice was observed by small animal live imaging system.The fluorescence expression and distribution of two kinds of cells in nude mice were observed.The morphology,structure and differentiation of iPS cells and A549 cells in tumor and organ were observed by tissue section method.RESULTS:The model of mouse subcutaneous tumor was successfully established,and the biological characteristics of A549 cell tumor were comprehensively understood.Vivo imaging system detects derived GFP expression in the liver of mice,and low or no expression of RFP expression in the liver.It is revealed that iPS cells tend to migrate toward the liver in the early tumor cell metastasis.In the observation of tumor tissue,it was found that cells with two proteins were expressed at the same time,indicating that two cells may develop in the process of tumor growth.