The Mechanism Of Apolipoprotein A-I Mimetic Peptide D-4F On Preventing Vascular Endothelial Cell Dysfunctions Induced By Contrast Media

Objective:The purpose of the present study is to investigate whether apolipoprotein A-I(apoA-I)mimic peptide D-4F could prevent contrast media-induced vascular endothelial cell dysfunctions and the related mechanism.Methods:HUVECs were isolated and cultured on the standard conditions.Cell migration was determined with the transwell chamber and scratch-wound assay.Angiogenesis was determined with the tube formation assay.Intracellular reactive oxygen species(ROS)production was assessed by ROS-mediated DCF fluorescence.Superoxide dismutase(SOD),hemeoxygenase-1(HO-1)and AMPK expression were measured by western blotting.Results:D-4F promoted the migration and tube formation of HUVECs.D-4F prevented contrast-induced apoptosis of HUVECs.D-4F remarkably inhibited ROS production and monocyte adhesion triggered by contrast media in HUVECs.D-4F promoted HO-1 and SOD expression in a dose and time dependent manner.D-4F up-regulated HO-1 expression through AMPK pathway.Conclusion:Apolipoprotein A-I mimetic peptide D-4F inhibited ROS production triggered by contrast media,and prevented the apoptosis and inflammation of HUVECs.D-4F promoted HO-1 expression through AMPK pathway,and could also upregulate the expression of SOD.In summary,D-4F protected endothelial cell from apoptosis and inflammation induced by contrast media,which might make D-4F to be a potient agent in improving contrast media-triggered endothelial injury.