The Preliminary Comparison And Quality Evaluation Of Metabolites Between Different Bombyx Batryticatus

An HPLC-MS based metabolomic method was employed to study metabolites difference between lab cultured bombyx batryticatus and bombyx batryticatus bought from pharmacies which were pruduced at different production places by different producers.From this research we can get a preliminary understanding about the metabolic influences exerted by different starins of Beauveria bassiana,different production areas and environments,and different processings of Bombyx batryticatus.In addition,we used the same method to identify the different metabolites between the peels of Bombyx batryticatus,Bombyx batryticatus without peels and Bombyx batryticatus without peeling.The result will contribute to provide a theoretical references for bombyx batryticatus production,products quality control,and processing of Bombyx batryticatusChinese patent medicine.1 Metabolome differences between lab cultured Bombyx batryticatus and Bombyx batryticatus of different producing area bought from the pharmaciesBombyx batryticatus,a traditional Chinese medicine oriental crude drug,is named for the dead and dried larvae of the silkworm Bombyx mori L.which was parasited to deth by Beauveria bassiana.Bombyx batryticatus is a valuable Chinese herbal medicine,is clinical ly used for treating headache,migraine,toothache,tonsillitis,convulsion,fever caused by exogenous pathogens,epilepsy,cough,asthma,exfoliative dermatitis,insomnia,thymoma,diabetes mellitus,urticaria,cerebral thrombosis,hyperlipemia,and other kinds of diseases.modern pharmacological studies showed that Bombyx batryticatus has anticoagulant,anti-thrombosis,anticonvulsant,anticancer,hypoglycemic lipidlowering and other effects.So far,domestic and foreign scholars have done a mass of analysis on the chemical composition of Bombyx batryticatus,the separation and purification of the active ingredients and the determination of the content and pharmacological effects of the herbal medicine,however,the metabolomic difference between different Bombyx batryticatus has not reported yet.In this study we use metabolomic method to study metabolome difference between lab cultured Bombyx batryticatus and Bombyx batryticatus of different producing area bought from the pharmacies.The results of principal component analysis showed that the metabolic difference between lab cultured Bombyx batryticatus and Bombyx batryticatus of different producing area bought from the pharmacies,as well as Bombyxbatryticatus from different producing area.We use Orthogonal Projection on Latent Structure-Discriminant Analysis was used to identify metabolome differences results revealed significant differences between different Bombyx batryticatus.The discriminating variations included pyruvic acid,GABA,proline,adenine,phenylalanine,folic acid,antibioticCV1,?-sitosterol,gingerenoneA,beauverolideB,bassianin,phosphatidylcholine(18:2/0:0),phosphatidylserine(18:2/0:0),palmitamide and so on.Metabolic pathways analysis revealed several pathway differences between different Bombyx batryticatus have most change of many metabolic pathways,such as purine metabolism,amino acid metabolism,fatty acid metabolism,glycolysis pathway and tricarboxylic acid pathway and some other metabolic pathways.Lab cultured Bombyx batryticatus contains higher concentration of GABA,ribulose,ammoniumtartarate,luteolin-7-glucoside,tryptophan,peonidin-3-O-beta-Dglucoside,oxytetracyclin,folicacid,prochlorperazinesulfone,quercetin,abyssinone,cimaterol,(2R,3R)-2,3-dihydroxybernsteinsaeure,2-methylpentanoic acid,trigonelline,antibiotic CV1,noformicin,cinobufagin,?-sitosterol,scoparin,glycyrrhetinic acid,methyloctacosanoate,pyroglutamic acid,phosphotidycholine(18:2/0:0),phenylalanine and so on.Above metabolites include bioactive compounds such as antioxidants and antibiotics,therefore,lab cultured Bombyx batryticatus may have more utilization value.But the reason why the commercial Bombyx batryticatus contains less bioactive compounds needs further research.2 Metabolomic difference between Bombyx batryticatus without peeling,the peels of Bombyx batryticatus,and Bombyx batryticatus without peelsOuter part of Bombyx batryticatus is skin of silkworm larvae and some mycelia of Beauveria bassiana.The inner part of Bombyx batryticatus is full of mycelia of Beauveria bassiana.However,the metabolites difference between the different parts are unknown.Clarify the difference can be helpful for rational utilization of Bombyx batryticatus.In this study we use metabolomic method to study metabolome difference among Bombyx batryticatus without peeling,the peels of Bombyx batryticatus and Bombyx batryticatus without peels.The results showed that there were significant differences existed between the samples.For example,peels of Bombyx batryticatus contain higher concentration of dimethirimol-6,7-dimethoxycoumarin,S-(hercyn-2-yl)-L-cysteine-S-oxide,O-succinyl-L-ho moserine,DL-leucyl-glycyl-glycine and so on.Bombyx batryticatus without peels contains higher concentration of N-demethyl-desepoxy-maytansinol,paecilomycins,telmisartan(514.18),farnesyl diphosphate and so on.Bombyx batryticatus without peeling containshigherconcentrationof phenylalanine,phosphoethanolamine,phosphatidylserine(16:0/0:0),cyclo(L-lle-L-val),tamoxifen,Iriomoteolide1 a,candesartan and so on.These differences indicate that there is different bioactive metabolites between the inner and outer parts of Bombyx batryticatus,and the different parts may have different utilization value.Therefore,it is worthy of in-depth study.