Research The Effect Of MnTBAP On The Occurrence And Development Of Postmenopausal Osteoporosis Basing On The Mechanism Of Oxidative Stress

Objective:With the aging of the population,senile osteoporosis and osteoporosis in postmenopausal women have become a common social problem,postmenopausal osteoporosis is more prominent.Research on postmenopausal osteoporosis has never stopped,while the mechanism is not clear.Standing on the important pathogenesis of oxidative stress,the main job of the reserach is to study the role of MnTBAP in the development of postmenopausal osteoporosis.Methods:This study is composed of animal experiment and cell experiment.In animal experiments,base on the ovariectomized osteoporosis model,the experimental animals were randomly divided and gived line modeling and drug intervention.,the animals were sacrificed after 6 weeks.We evaluate the expression level of Mn-SOD in the bone tissue of the each groups by the western-blot technique.The levels of AOPP(advanced oxidation protein),MDA(malondialdehyde)and SOD(superoxide dismutase)in serum and bone homogenate of each group were detected,the corresponding changes of oxidative stress were evaluated.Simultaneous detection of BMP-2(bone morphogenetic protein-2)in bone tissue by western-blot and immunofluorescence monitors the changes of the expression level of the functional protein BMP-2 inducing osteogenic differentiation.Finally,micro-CT was used to detect the changes of bone mineral density and bone microstructure.In experiments of cells,the bone marrow mesenchymal stem cells were induced to differentiate into osteoblasts under the same oxidative stress environment.In this process,different concentrations of MnTBAP were given The expression of Mn-SOD in cells and the level of AOPP,MDA and SOD in cell homogenate was detected and used to evaluate the effect of MnTBAP on the oxidative stress..At the same time,western-blot was used to detect the expression level of BMP-2 in cells.Finally,osteoblast specific staining was performed to evaluate the effect of MnTBAP on osteogenic differentiation under oxidative stress.Results:In animal and cell experiments,MnTBAP intervention significantly increased the expression levels of Mn-SOD,which was negatively correlated with mitochondrial oxidative stress.The levels of oxidative stress in the corresponding cells and the whole body were reduced,and the expression level of the functional protein BMP-2,which had strong osteogenic induction function,was increased.The degree of differentiation showed by the osteoblast specific staining,the bone mineral density and bone microstructure were improved.Conclusion:MnTBAP can reverse the oxidative stress level of the whole cell and the whole body by adjusting the mitochondrial oxidative stress,and the direct effect is that the expression level of BMP-2 is increased.The degree of differentiation of osteoblasts was enhanced,the effect of osteogenesis was enhanced,and the development of osteoporosis was inhibited.MnTBAP,which has been applied to other research fields,is an effective drugs for postmenopausal osteoporosis.