MiR-873 Promotes Cell Proliferation,metastasis And Invasion By Directly Targeting TSLC1 In Hepatocellular Carcinoma

Background:Hepatocellular carcinoma(HCC)is the most frequent type of primary liver cancer with it's third mortality in the world.MicroRNA is a class of endogenous,single-stranded,short noncoding RNA,which regulates preliferation,opoptosis and metastasis in numbers of tumors.Methods:The expression of miR?873 in HCC tissues and cell lines was detected by RT-PCR.CCK-8 were used to examine cell growth,flow cytometry used to detect cell cycle,and transwell used for metastasis and invasion.Luciferase assays were performed to assess the target of miR-873.RT-PCR,western blot and immumohistochemical staining were used to detected the expression of TSLC1 in HCC tissues and cell lines.Western blot was used to detected the level of signal pathway related proteins.Results:We showed that miR-873 was upregulated in HCC tissues and cell lines compared with the normal control.Knockdown of miR.873 inhibited the growth,metastasis and invasion of HepG2 and accelerated G1 phase arrest.But overexpression of miR-873 had the opposite effect.The dual-luciferase reporter assays revealed that TSLC1 was a novel target of miR-873.Further study showed TSLC1 was decreased in HCC tissues and cell lines.There was a negative correlation between the expression of TSLC1 and miR-873.The effect of miR-873 overexpression was neutralized by TSLC1.We also found that miR-873 actived JAK2/STAT3 signal pathway to promote HCC.Conclusions:MiR-873 was increased in HCC.MiR-873 promoted HCC cells proliferation,metastasis and invasion through targeting TSLCI.The potential mechanism might be the activation of JAK2/STAT3 signal pathway.