Regulation Of All-trans Retinoic Acid On Differentiation Of Neural Stem Cells In Adult Mice

Objective:This experiment aimed at exploring the effects of all-trans retinoic acid(ATRA)on the differentiation direction,signal transduction pathway and cytochrome P450(CYP450)family of neural stem cells(NSCs)in adult mice.Methods:NSCs were isolated from the sub-ventricular zone(SVZ)of adult mice brain and cultured.ATRA were used to detect the effect on proliferation of NSCs.Flow cytometry(FCM)was used to check the NSCs differentiation markers.Real-Time PCR for gene expression of CYP450 super-family,while ELISA for the activity of cytochrome P450 reductase(CPR).The expressions of CPR,P38 and P-P38 were detected using Western blot.Results:1.ATRA reduced the number of neurospheres,which meant inhibition on the proliferation of NSCs.2.ATRA promoted the differentiation of NSCs,mainly differentiated into neurons,and some differentiated into astrocytes,with less differentiation of oligodendrocytes.3.ATRA promoted the expression of CYP26 family and the expression,activity of CPR,together with the expression of P-P38.Conclusions:ATRA can inhibit the proliferation of NSCs in adult mice and promote its differentiation into neurons and astrocytes.During this process,the CYP26 family and CPR play an important role.This regulation may be conducted through the P38MAPK pathway.