Endostatin Combined With BFGF Effect On Hypertrophic Scar In A Rabbit Ear Model

Objective: To investigate the effects of endostatin combined with basic fibroblast growth factor(bFGF)on wound of rabbit ears hypertrophic scar and its mechanism,and in order to provide a new treatment method and prevention of pathological scar.Methods: Rabbit models of hypertrophic scar were established.After the model was successfull y established,12 rabbits were randomly assigned to 4 groups,and each rabbit ear is designed for 6 wounds,with a total of 144 wounds.They are group A(bFGF group),group B(endostatin group),group C(bFGF+end-ostatin group),and group D(saline group).Packet processing is as foll-ows: In group A and group C topical bFGF was applied to external scaboff.About 21 st day postwounding,intraperitoneal injection of endostatin was performed each day in group B and group C,group D was treated with an eaqual volume of normal saline;Rabbits were euthanared and their scars were harvested for histological and proteomics analysis on 42 nd day after being postwounded.On the 14 th day,21 st days,28 th days,and42 nd days to observe the wound and scar.Scar thickness should be recorded.On the 42 nd day after surgery;in each group,rabbit ear scar tissues were collected,using hematoxylin eosin staining to observe the morphological changes of scar tissue,immunohistochemical method to detect microvessel marker CD34 expression and bFGF,Western blot method to de-tect the expression of bcl-2.The expression of CD34 and bFGF was observed by microscope.The absorbance value of positive area per unit are a was measured by Image-pro plus 6.0 image analysis software system,and the expression intensity of CD34 and bFGF was replaced by IOD value.Each immunohistochemical stain was to be observed under high power microscope,and 5 visual fields were selected.The IOD values of each field of each specimen were measured respectively,and the average number of 5 fields of vision was taken as the IOD value of the specimen,and the comparison was made.Results:The results of histomorphologic al observation showed that the hypertrophic scar in the experimental C group was obviously reduced:the scar color decreased obviously,and the collagen in the scar tissue was loose and arranged neatly,and there was a significant difference compared with the group A and the group B.The results of the IOD value of CD34 in each group were: A(22.84+0.3),B group(12.7+0.53),C group(5.93+0.57),D group(41.96+3.82).That is:group C<B group<A group<D group,and when group C compa-red with other groups,P<0.05.The results of the IOD values of bFGF were:group A(133765.37+5316.70),group B(296209.91+22961.17),group C(552960.53+56787.72),group D(58571.32+2414.95).That is:group C>gr-oup B>group A>group D,and when group C compared with other groups,P<0.05.The results of Bcl-2 protein immunoblotting(Western blot):group A(0.341+0.029),group B(0.201+0.015),C group(0.076+0.015),D group(0.539+0.063).That is: C group <B group <A group <D group,and when groupC compared with other groups,P<0.05.Conclusion: 1.Intraperitoneal injection of endostatin has inhibitory effect on vascular endothelium and all eviating scar hyperplasia;2.Bovine basic fibroblast grow-th factor can promote wound healing,and it may reduce scar angiogen-esis to reduce scar hyperplasia,but the effect is significantly weaker than endostatin;3.Endostatin combined with recombinant bovine basic fibro-blast growth factor(bFGF)may produce synergistic effect and two of them can inhibit microvasculature.The mechanism may be that endostatin and recombinant bovine basic fibroblast growth factor synergistically inhibit the expression of Bcl-2 gene,and then they directly or indirectly ac-celerate the apoptosis of endothelial cells and fibroblasts,and reduce the formation of cytokines,thus reducing scar proliferation.