The Correlation Between The BDNF Gene Polymorphism And Resting-state Function MRI Of Brain In Schizophrenia

BackgroundSchizophrenia is one of the severe mental disorder.At present,schizophrenia is recognized as a complex genetic disease.Due to the complexity of clinical symptoms,genetic phenotypic characters of schizophreniais are determined by coefficient minor responses of multiple genes,and vulnerable to environmental impact,Therefor,genetic studies of schizophrenia generally are poor reproducibility.Fortunately,the technology that combined image and genomics can explore and evaluate the effects of the related gene on function of brain,so as to understand the influence of the genes on the behavior in schizophrenia.Hence,image genomics methods were used to study the pathogenesis of schizophrenia.ObjectiveTo search the changes of brain imaging changes specific to schizophrenia,and to explore the correlation between the SNPs of BDNF and resting-state function MRI of brain in schizophrenia.MethodA hundred patients with schizophrenia and 114 normal controls were enrolled,the BDNF polymorphism(rs2030324 andrs11030101)and allele frequency were genotyped by sequencing the products of PCR.Genotype and allele frequencies were compared between patients and controls to assess the association to schizophrenia.Symptoms were assessed using the PANSS scale,then analysis the relationship between the score of PANSS and the polymorphism of rs2030324 and rs11030101.Collection the resting state fMRI scanning of40 patients of schizophrenia and 40 healthy subjects and analysis their differences of ALFF.Finally,the imaging data of case control two groups were divided into different subgroups according to the positive loci genotyping,researching the differences between their brain imaging,to find the correlation between the BDNF gene polymorphism and resting-state function MRI of brain in SchizophreniaResultThere was statistically significant in the allele distributions of rs2030324(?~2=4.481,P=0.034)and in the genotype and allele distributions of rs11030101(?~2genotype=6.149,P=0.046,?~2allele=4.486,P=0.0276)between schizophrenia patients and controls.There was statistically significant between schizophrenic patients and controls in the distribution of implicit model in rs11030101(?~2=5.230,P=0.022).The score of PANSS negative symptoms of patients with SNPs rs11030101 different genotypes showed that,A/T was the highest and T/T minimum,the difference had statistical significance(F=4.868,P=0.010).FMRI data analysis showed that schizophrenia patients are significantly increased ALFF in the right orbit frontal cortex and decreased ALFF in the right middle cingulum.Significant diagnosis×rs2030324 genotype interaction was noted in the right orbit frontal cortex(P<0.05,corrected)and the AA/AG genotype group had significantly lower ALFF values in the right orbit frontal cortex than the GG group(T=-5.19,P<0.05)among controls.ConclusionBDNF gene rs2030324 and rs11030101 are associated with han schizophrenia.Allele A may be the susceptibility alleles of schizophrenia.The SNP of rs11030101 is associated with the clinical features of schizophrenia.There is association of BDNF and abnormal brain activities in resting-state patients with schizophrenia.