Study On Silk Scaffolds With Controllable Mechanical Properties And Biocompatibility

Stem cells could respond to the mechanical properties of materials and transformed them into functional cells that match mechanical properties to achieve directional differentiation.Compared with hard tissues,the regeneration of soft tissues required biomaterials with softer mechanical properties.Meanwhile,The fine mechanical tunability provided a feasible way to improve cell proliferation and enhancing neovascularization.The existing materials had high mechanical properties and could be applied to hard tissues and some soft tissues.However,it could not be applied to soft tissues that required softer mechanical properties and could not achieve fine control under mechanical properties.How to prepare materials that could adjust lower mechanical properties with finely tunable and satisfied the requirements of tissue repair is the hot topic in current material research.Compared with other materials,The amorphous silk nanofiber solution had obvious advantages because of its biocompatibility,biodegradability and processability.This particle use amorphous silk fibroin nanofiber solution as element.On the one hand,the self-assembly process of silk fibroin was regulated to get insoluble silk fibroin scaffolds with stiffness at 1-4k Pa bytemperature-induced.Its basic properties and biocompatibility were also studied.The results showed that the conformation,modulus and degradability of porous sponge could be changed by dynamic factors.Both in vivo and in vitro results showed good cytocompatibility and histocompatibility,and could be used in the field of soft tissue repair.On the other hand,glycerin-induced and temperature-induced were used to further reduce mechanical properties for nerve tissue repair,and studied the conformation,biodegradability and mechanical property of silk fibroin scaffolds.The results showed that insoluble silk fibroin scaffolds with stiffness below 1k Pa were prepared by glycerin-induced and temperature-induced,which might be better used in nerve tissue repair.In summary,in this study,the silk fibroin scaffolds with controllable conformation and mechanical properties were prepared by an effective and simple method.These scaffolds enriched the breadth of application of silk fibroin scaffolds in tissue engineering and provided a promising strategy for soft tissue repair and regenerative medicine.