Effect Of A Novel Focal Adhesion Kinase Inhibitor TAE226 On The Growing Of Human Oral Squamous Cell Carcinoma Cell Line In Vitro

objective:To study the effects of a novel adhesion kinase inhibitor TAE226 on the proliferation,migration and invasion of HSC-3 and HSC-4 cell lines in vitro,and to explore the mechanism of its effect on oral squamous cell carcinoma.Methods: 1.CCK-8 assays was used to detect the effect of TAE226 on the proliferation of oral squamous cell carcinoma cells: The oral squamous cells were co-cultured with TAE226 at the concentration of 1umol/L,5umol/L,10umol/L,20umol/L for 12,24,36,48,60,72 hours.The OD value of the cells in each group were measured by enzyme labeling and the inhibition of the cell proliferation rate caused by TAE226 at different concentrations and times were calculated at last;2.Transwell assays were performed to investigate the cell migration and invasion abilities of TAE226.The cells were treated with different concentrations of TAE226 for 24 hours after Serum-free culture of oral squamous carcinoma cells for 12 h.The migration and invasion rate of each group were calculated by counting the number of transmembrane cells.3.The effect of TAE226 on the migration of oral squamous carcinoma cells was detected by scratch test.The cells were co-cultured with TAE226 at the concentration of 1umol/L,5umol/L,10umol/L,20umol/L for 12,24,48 hours respectively.The mobility of each group was calculated by comparing the changes of scratch width;4.All data were analyzed by SPSS17.0 software,and they expressed by.Experiment groups were compared with single factor analysis of variance(one-way ANOVA).There are significant differences once P<0.05.Results:1.The results of CCK-8 proliferation test showed that Compared with the control group,the proliferation of HSC-3 and HSC-4 cells treated with different concentrations of TAE226 was inhibited(p < 0.05).When the concentration of TAE226 was 1 ?mol/L,there was no significant difference between the two groups at 12 h.However,with the prolongation of time,the difference was statistically significant(p < 0.05).With the increasing of TAE226 concentration,the inhibitory effect of TAE226 on HSC-3/HSC-4 cells proliferation was gradually enhanced,and at the same concentration,with the prolongation of TAE226 action time,Its inhibitory effect on cells was enhanced(p < 0.05).2.Transwell migration experiment results: Compared with the control group,the number of transmembrane cells in the experimental group decreased significantly.With the increase of the concentration of TAE226,the inhibitory effect of transwell migration on the cells was gradually enhanced(p < 0.05).3.The result of scratch test: compared with the control group,the number of transmembrane cells in the experimental groups were lower than that in the control group remarkably.The cell migration was inhibited by different concentrations of TAE226 at different concentrations.The results showed that the inhibitory effect of 1 ?mol/L and 5 ?mol/L TAE226 on cell migration was negatively correlated with the dose of TAE226.However,there was no significant difference between the 5 ?mol/L and 10 ?mol/L groups in the inhibition of cell migration.After the cells were treated with TAE226 for 12 h and 24 h,the inhibition rate of migration was found to be time-dependent(P < 0.05)?4.Transwell invasion assay: Compared with the control group,with the increase of TAE226 concentration,the number of HSC-3 and HSC-4 transmembrane cells decreased,and the cell invasion rate decreased.Conclusions: 1.TAE226 could effectively inhibit the proliferation of HSC-3/HSC-4 cells,and with the increase of its concentration,the inhibitory effect was gradually enhanced.With the increased of time in the same concentration of TAE226,The inhibitory effect was gradually enhanced.2.TAE226 could effectively inhibit the migration of HSC-3/ HSC-4 cells.The inhibitory effect increased gradually with the increase of TAE226 concentration,and increased with the prolongation of the time of action.The inhibitory effect was also enhanced.3.TAE226 could effectively inhibit the invasion of HSC-3/HSC-4 cells in a dose-dependent manner.4.This study will provides a theoretical basis for the treatment and mechanism of oral squamous cell carcinoma.In a word,The cell proliferation,migration and invasion of oral squamous carcinoma cell lines HSC-3 and HSC-4 can be inhibited effectively.