Intervention Of Vitamin D In Regulating The Migration And Invasion Of Breast Cancer Cells By Regulating Ras Expression

?Objective?To verify that vitamin D inhibits breast cancer cells MCF-7(VDR+/ER+)and MDA-MB-453(VDR+/ER-);To investigate the correlation between the inhibition of vitamin D in breast cancer cells and the RAS/MAPK/ERK signaling pathway.?Methods?1.Subcultivation of breast cancer cells MCF-7(VDR+/ER+)and MDA-MB-453(VDR+/ER-);2.Constructed of Ras overexpression vector;3.Cells were divided into blank group,control group and experimental group for corresponding treatment;4.After cultured cells to logarithmic phase and interaction for 48 hours,scratch and tanswell invasion assays were used to detect the migration and invasive ability of the two cells.?Results?1.1,25-(OH)2D3 inhibited the migration and invasion of breast cancer cell lines MCF-7(VDR+/ER+)and MDA-MB-453(VDR+/ER-)(P < 0.05).2.Ras overexpression in breast cancer cell lines MCF-7(VDR+/ER+)and MDA-MB-453(VDR+/ER-),1,25-(OH)2D3 reversed the inhibition of migration and invasion in breast cancer cells(P < 0.05).?Conclusions?1.1,25-(OH)2D3 inhibit the migration and invasion of breast cancer cells lines MCF-7(VDR+/ER+)and MDA-MB-453(VDR+/ER-).2.In vitro cell experiments,1,25-(OH)2D3 may inhibit the migration and invasion of vitamin D receptor-positive breast cancer cells via RAS/MAPK/ERK signaling pathway.