| Objective:To investigate the radiosensitization effect of celecoxib on human nasopharyngeal carcinoma CNE-2 cells and its synergistic effect with CMNA,and to explore its possible mechanism.Methods:(1)MTT was applied to detect the inhibitory effect of celecoxib and CMNA on the proliferation of CNE-2 cells.The inhibition curve of proliferation was plotted to determine the effect of celecoxib and CMNA.(2)The clone formation assay was divided into four groups: Simple irradiation group(IR),Celecoxib + radiotherapy group(C+R),CMNA + radiotherapy group(G+R),celecoxib + CMNA + Radiotherapy group(C + G + R).Survival fraction(SF)of each group was calculated to build a target model to fit the cell survival curve to calculate the sensitization enhancement ratio(SER),to detect radiosensitivity of CNE-2 cells.(3)The cell cycle distribution and apoptosis rate of celecoxib plus CMNA on CNE-2 cells after radiation were detected by flow cytometry(FCM).Results:(1)After treated with different concentrations of celecoxib for 24 and 48 hours,CNE-2 cells inhibited the proliferation of CNE-2 cells in a dose and time-dependent manner as the drug concentration increased and the action time prolonged(P < 0.01).To select the concentration of 20μg / ml as the optimal working concentration.CNE-2 cells were treated with different concentrations of CMNA for 24 h and 48 h,the cells had no significant inhibition of proliferation(P> 0.05).The concentration of 25μg / ml was chosen as the optimal working concentration.(2)In the 0,2,4,6Gy irradiation range,the SF in the(C+R)group and(G+R)group were lower than that in the(IR)group,and SF decreased with the increase of the radiotherapy dose(P< 0.01).The SF in the(C + G + R)group was more lower obviously than the(IR),(C+R)and(G+R)groups,and SF decreased with the increase of the radiotherapy dose(P< 0.001).D0 in(IR),(C + R),(G + R)and(C + G + R)groups were 4.45,3.98,4.10 and 2.60.Compared with(IR)group,the SER of(C + R),(G + R)and(C + G + R)groups were 1.12,1.09 and 1.71.(3)Compared with IR group,the cell cycle distribution indicated that CNE-2 cells treated Drugs combined with radiotherapy increased the proportion of G0 / G1 phase and decreased the proportion of S phase.(P < 0.01).(C + R),(G + R)and(C + G + R)groups can induce apoptosis of CNE-2 cell.(P < 0.01);Compared with(C+R)and(G+R)groups,the apoptosis rate of(C + G + R)group was more obvious.(P < 0.01).Conclusions:1.Celecoxib significantly inhibited the proliferation of CNE-2 cells in a dose and time-dependent manner;The CMNA did not significantly inhibit the proliferation of CNE-2 cells.2.Celecoxib and CMNA can increase radiosensitivity of CNE-2 cell,the two drugs synergistic synergistic effect.The combination of two drugs have synergistic radiosensitization.3.Celecoxib,CMNA and the combination of the two drugs may increase the radiosensitivity of CNE-2 cells by inducing apoptosis,inducing cell cycle arrest and blocking DNA synthesis. |
