Relationship Between PRMT2 And HMGA1 And Its Effect On Glycolysis Of Breast Cancer Cell Line MCF-7

Objective To explore the relationship between protein arginine methyltransferase2(PRMT2)and high-mobility group A1(HMGA1)and their effect on the glycolysis metabolic pathways of breast cancer MCF-7 cells.Methods1.The expression of PRMT2 and HMGA1 in breast cancer cell line MCF-7,T47 D,and MDA-MB-231 were detected by Western blot(WB).2.The co-immunoprecipitation was used to preliminarily study the relationship between PRMT2 and HMGA1 in MCF-7 cells.3.Establish a breast cancer MCF-7 cell line that stably low expresses PRMT2,transfect the Lac Z-mi RNA empty plasmid group into a negative control group,and transfect PRMT2-mi RNA1 and PRMT2-mi RNA2 into experimental group 1 and experimental group 2,respectively.Western blot(WB)was used to detect the expression of HMGA1 in negative control and experimental groups.4.By measuring the glucose consumption of MCF-7 cells in the negative control group and the experimental group,initially determining its effect on glycolysis of MCF-7 cells.5.The expression of glycolytic enzyme in MCF-7 cells which stably expresses PRMT2 in a low level and its subcellular structures,were detected by Western blotting.The effect of PRMT2 on the expression of glycolytic enzymes(PFKP,Enolase-2,Hexokinase 1 and PKM2)in breast cancer MCF-7 cells was clearly underlined.Results1.Western Blot results showed that PRMT2 was highly expressed in breast cancer MCF-7 cells and T47 D cells,and low expression in MDA-MB-231 cells;HMGA1 was lowly expressed in breast cancer MCF-7 cells and T47 D cells,and in high expression in MDA-MB-231 cells,the difference was statistically significant(P<0.001).2.The result of co-immunoprecipitation showed that there was no interaction between PRMT2 and HMGA1 in MCF-7 cells.3.Western Blot analysis showed that the expression of HMGA1 was increased in breast cancer MCF-7 cells stably expressing low PRMT2.4.The results showed that compared with the negative control group,the glucose consumption of MCF-7 cells with low expression of PRMT2 was increased,with statistical significance(P<0.05).5.Western Blot analysis showed that the expression of Enolase-2,PFKP and PDHK1 in MCF-7 cells with stable expression of PRMT2 was increased,and the difference was statistically significant(P<0.05).However,no significant changes in expression of PKM2 and Hexokinase 1 were observed.The expression of Hexokinase 1 and PKM2 in the nucleus of the experimental group increased,and the expression in the cytoplasm decreased.The difference was statistically significant(P<0.05).Conclusions1.Low expression of PRMT2 can increase the expression of HMGA1 in MCF-7 cells.2.Low expression of PRMT2 can promote glycolysis of breast cancer MCF-7 cells.