Clinical Analysis Of Influencing Factors Related Genes And Their Mutations Status In Patients With Non-small Cell Lung Cancer

ObjectiveTo explore the mutation frequency of related genes and influencing factors in non-small cell lung cancer(NSCLC).MethodsIn this study,the next generation sequencing(NGS)technique(Next-generation sequencing NGS)was used to detect and analyze the related genes in the samples of enrolled patients.Patients with NSCLC admitted to the first affiliated Hospital of Soochow University from September 2016 to December 2017 were studied.The expression and mutation of EGFR,TP53,ALK,ERBB2,KRAS,MET,ROS1,BRAF and RET were detected,and their biological behaviors were discussed and analyzed from 6 different aspects,including sex,age,smoking history,pathological type,clinical stage and family history of cancer.At the same time,the correlation of the above mutation genes and KRAS were compared and summarized.A simple analysis was performed among 28 patients with tumor mutation load(TMB)detection.ResultsIn the 235 patients of this study,the ?2 test showed that the mutation of EGFR gene was correlated with sex and pathological type;mutation of TP53 gene was correlated with sex,smoking history,pathological type and so on;mutation of ALK fusion gene was correlated with pathological types;mutation of KRAS gene was correlated with pathological type and family history of cancer.There was a negative correlation between the positive rate of driver gene mutation and the high level of TMB.In this study,four genes(EGFR,TP53,ALK,KRAS)were analyzed by binary logistic regression analysis.The results showed that sex and pathological type were the susceptible factors of EGFR gene mutation;sex,age and pathological type were the susceptible factorsof TP53 gene mutation;pathological type and family history of cancer were the risk factors of KRAS gene mutation.The mutation of KRAS gene can coexist with the mutation of TP53,EGFR,ALK,and MET,and the probability of combining TP53 mutation is higher.ConclusionThe incidence of EGFR gene mutation was high in female and adenocarcinomas.The mutation of TP53 gene was significantly higher in male and squamous cell carcinoma patients,and it was positively correlated with age.The pathological type of ALK gene mutation was adenocarcinomas.The mutation of KRAS gene was found in lung adenocarcinoma patients,and the family history of cancer was the predisposing factor.The mutation of KRAS gene can exist at the same time with other gene mutations.There was a negative correlation between high TMB level and positive driving gene mutation.