Exploration Of Treatment Of Glioma By Transcranial Direct Current Stimulation

ObjectiveTo explore the effect of transcranial direct current stimulation in the treatment of gliomaMethods1.The C6 glioma cells were cultured in vitro,and after the stable passage,the logarithmic growth stage cells were transplanted subcutaneously in nude mice.2.After feeding the tumor bearing nude mice and observing the growth and stability of the subcutaneous tumor tissue,continuous direct current stimulation was given to the nude mice.3.Observe the size of subcutaneous tumor tissue in nude mice and analyzed the growth of tumor after direct current stimulation.4.The tumor tissue was stained by HE to observe the cell morphology,and the effects of electrical stimulation on the cells were analyzed after the nude mice were killed.Results1.The C6 glioma cells were successfully cultured in vitro.2.A stable subcutaneous glioma model of nude mice was established.3.Completed the design and installation of the DC electric stimulation device.4.By observing the volume change of tumor,the growth of tumor after DC stimulation is obviously slower than that of tumor tissue without electrical stimulation.It is proved that DC stimulation has certain effect on slowing down glioma growth.5.Compared with the non electrified group,there was no significant difference between the cell morphology and the non electrified group after HE staining.Conclusion1.Direct current stimulation technology plays a certain role in slowing down the growth rate of glioma,providing a basis for further expanding the application of direct current stimulation technology in nervous system diseases.2.After DC stimulation,the morphology of C6 glioma cells did not change significantly,and no specific manifestations such as apoptosis were observed.The specific mechanism still needs further exploration.