| The development of tissue engineering has provide a technology of tissue regeneration for tissue injury or trauma,and it will change the traditional theory of “traumatic repair wound” into the clinical treatment of non-injury repair.It is most important cells were the bone mesenchymal stem cells and endothelial cell,which play an important role in tissue engineering neovascularization.BMSCs as the most widely used seed cell in tissue engineering provides the initial factor for the tissue construction,and in constructs material with cell activity in vitro,made it possible to repair the large traumas.Meanwhile,as the carrier and executor for tissue engineering neovascularization,how to rapidly activate the static endothelial cells,as soon as possible to complete the implanted tissue revascularization,which is great significantly for tissue engineering.It has reported that BMSCs play a therapeutic role in repairing damaged organs or tissues through multiple targets and multiple mechanisms.BMSCs can secrete a variety of paracrine factors,including cytokines,chemokines and trophic factors,which play different roles in different cells.Materials composited with BMSCs were transplanted into the position of wound,causing the growth of blood vessels and accelerating the repair process of trauma.However,the mechanism is poorly characterized.It's important to investigate whether BMSCs be able to secrete some of factors by the paracal secretion,and how to regulate the EC to promote vascularization.In the first part,we utilize the indirect co-culture system to simulate the interaction of BMSCs and RAOEC and investigate the effect of factors released from BMSCs by paracrine on the EC functions in vitro,and the Exosomes in supernatant of co-culture system were analyzed by TMT Labeling Quantitative Proteomics.We demonstrated that the Proliferation,migration,invasion,and vessel formation of ROAEC were higher than the group without BMSCs cultured.We further identified the Exosomes of co-culture group and the bioinformatics shown that BMSCs have an effect on Extracellular matrix binding,adhesion,and transduction of signal pathways of ROAEC.The differentiated protein was screened by proteomics also establishes the basis for the next research.Our research group is committed to the study of bone tissue engineering by constructing a bone defect animal model and observed the repair effect of bone defect during bone repair period.Most fundamental studies evaluate the process of bone repair by means of bone structure regeneration assessments,such as radiologic assessment and histological staining,which reflected the deposition of inorganic salts and collagen during bone repair period.Bone is a complex organ with numerous functions,including hematopoiesis,regulation and storage of key minerals,protection of vital life-sustaining organs,and facilitation of locomotion.The biomechanical properties of bone are of the utmost importance since the primary function of long bone is load-bearing.Hence,the recovery of the mechanical integrity of the healing fracture callus is arguably the most important metric in long bone.For basic bone research,EGFP transgene rats are often used as sources of cells and gene reporters and for some disease models,while the ability of bone repair has not been reported.In the second part,we utilized the EGFP transgene rats and wild-type Sprague-Dawley(SD)rats as experimental subjects and created the femoral drilling defect model.Compared the structural regeneration and functional regeneration of EGFP transgene rats and wild-type SD rats during the bone-repair process at the same repair time point.The result was deconstructed that the structural regeneration and functional regeneration have reflected the bone repair effect at the respectively aspects,and suggested that biomechanical functional measurement is more sensitive than structure regeneration measurement,which enriched the fundamental research of evaluation the repair effect after bone defect. |
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