| Objective 1.Screening of MTHFR gene C667 T polymorphism and CYP2C19 polymorphisms were distributed among the han and non-chd populations in guangdong province.2.To study the correlation between CHD population and MTHFR gene C667 T polymorphism and CYP2C19 gene polymorphism in guangdong province.3.To observe the correlation between MTHFR gene C667 T polymorphism and CYP2C19 gene polymorphism in the main adverse cardiovascular events in CHD group.Methods 1.To collect the coronary angiography of the second affiliated hospital of guangzhou medical university and the han patients in guangdong province with MTHFR and CYP2C19 gene detection.The condition was divided into normal group,hypertension group and coronary heart disease group.The basic data of the three groups were collected: age,sex,height,weight,blood lipid level(TC,TG,LDL),smoking,diabetes mellitus,family history of cardiovascular disease,etc.MTHFR(C677T)gene detection using pcr-chip hybridization method;DNA microarray chip method was used for the detection of CYP2C19 gene.Major adverse cardiovascular events in the coronary heart disease group were conducted by telephone,re-admission record or outpatient follow-up for 3-12 months.2.MTHFR(C677T)gene detection using pcr-chip hybridization method;DNA microarray chip method was used for the detection of CYP2C19 gene.Major adverse cardiovascular events in the coronary heart disease group were conducted by telephone,re-admission record or outpatient follow-up for 3-12 months.Results 1.Through the comparison of three groups of basic clinical data,can be concluded that CHD group in terms of age,smoking history,history of diabetes and high blood pressure in the normal group group significantly increased(P < 0.05),with statistical differences.2.The frequency distribution of CC,CT and TT at the C677 T site of the MTHFR gene in the three groups was statistically different from that of C and T allele.(2 =17.867,P =0.001;2 =15.258,P value <0.001);Among them,TT,CT genotype frequency and T allele frequency of coronary heart disease group were significantly higher than those of hypertension group and normal group(P<0.05),and the difference was statistically significant.After Logistic regression analysis and correction of relevant clinical indicators,TT genotype was a risk factor for coronary heart disease.(OR=2.725,95%CI: 1.072-6.961).3.There were no statistically significant differences in the frequency distribution of the genes in the G636 A locus of CYP2C19 gene in the three groups and the frequency distribution of G and A allele in G636 A sites and G681 A sites.(P > 0.05)4.Patients were followed up for 7(3-12)months after discharge in coronary heart disease group.A total of 99 patients with MTHFR gene were successfully followed,and 93 were CYP2C19.Upon examination,have adverse cardiovascular events in patients with recurrence of frequency,in comparison with patients with no recurrence frequency in MTHFR C677 T in CC,CT,TT three C,T allele and genotype distribution differences had no statistical significance(P > 0.05);In G636 A CYP2C19 gene locus on GG,GA three G,A allele and genotype distribution difference was statistically significant(P < 0.05),in GG,GA,AA G681 A sites with three kinds of G,A allele and genotype distribution difference was not statistically significant(P > 0.05).Conclusion 1.TT,CT genotype and T allele were closely related to CHD at the MTHFR gene C677 T site in the han population in guangdong province,and TT genotype was a risk factor for coronary heart disease in patients with hypertension.However,it was not found to be related to the recurrence of adverse cardiovascular events.2.The han nationality in guangdong G636 A CYP2C19 gene locus on coronary heart disease(CHD)the crowd A allele is associated with adverse cardiovascular events again hair,may affect the han nationality in guangdong coronary heart disease group efficacy of clopidogrel response sex;Genotypes and alleles at G681 A were not associated with adverse cardiovascular events. |
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