In Vitro Study Of Taurocholic Acid Inhibiting Hepatoma Cell Growth

Cholestatic liver disease,which may lead to liver cancer development,is closely associated with a variety of bile acids.However,the mechanisms of some bile acid acting on the liver during cholestasis and cholestatic liver diseases transforming into liver cancer remain unclear.Taurocholic acid（TCA）is highly elevated in cholestatic liver cirrhosis patients. Therefore,TCA can be used as an important marker for the diagnosis of cholestatic diseases.However,the mechanisms of elevated TCA content involved in this process are unclear.In addition,the effects of TCA on hepatoma cells are mainly focused on short-term studies,no significant changes in cell viability,and lack of studies on the effects of normal hepatocytes.In this study,the human normal hepatocyte THLE-2 was selected as the experimental control group model,and human hepatoma cell HepG2 was used as the experimental test group model.（1）To investigate the cell activity of TCA after long-term intervention with TCA in short-term interventional cells and in vitro with simulated cholestatic liver disease.（2）To test the apoptosis of TCA after a long-term intervention with TCA for short-term interventional cells and in vitro with simulated cholestatic liver disease,and to observe the apoptosis status;（3）To explore the mechanism of TCA short-term interventional cells and the in vitro model of TCA long-term interventional cells in the concentration of cholestasis liver disease from the level of relevant gene expression,intracellular reactive oxygen species and Ca²⁺ production.