Effect Of Silencing FoxM1 On Proliferation, Migration And Invasion Of Papillary Thyroid Carcinoma Cells

Objectives Through the discussion of application of liposome Lipofectamine 2000 as carrier of forkhead box M1(FoxM1)siRNA in human thyroid papillary carcinoma cell line K1,the transcription factor forkhead box M1(FoxM1)silenced,observation of human thyroid papillary carcinoma cell proliferation,migration and invasion of the migration effect.Methods Standardized training of papillary thyroid carcinoma cell line K1 cells grow to logarithmic phase in strict accordance with the use of si RNA-FoxM1 instructions,the expression in human thyroid carcinoma K1 cells by Western blot assay with FoxM1-siRNA;liposome lip-2000 FoxM1-siRNA and control siRNA respectively from human thyroid carcinoma cell line K1,as transfection and nonsense sequence group,another K1 cells added PBS solution as the blank control group;MTT was used to detect the packet after each cell proliferation;migration ability with each cell monolayer cell scratch assay after grouping;detect the invasive ability of each group cells by Transwell.Results 1 Fluorescent siRNA was transfected into K1 cell 48 h by liposome Lipofectamine 2000.The ratio of the number of transfected siRNA into total cell number was transfected efficiency under fluorescence microscope.The transfection efficiency of three times proved that the transfection efficiency could reach 80%.2 The results of immunoblotting showed that FoxM1-siRNA reduced the expression of FoxM1 protein.3 MTT experiment results showed that at three time points of 24 h,48h and 72 h,the proliferation ability of transfected cells was significantly lower than that of blank control group(P < 0.05).4 Single cell scar injury repair experiments showed that the migration ability of the transfected group was lower than that in the non significant sequence group and the blank control group(P < 0.05).5 Transwell cell invasion test showed that the number of cells in the transfected group was significantly lower than that in the blank control group,and the number of cells reaching the upper chamber was significantly different(P < 0.05).Conclusions 1 After transfecting siRNA-FoxM1 into K1 cell lines,the apoptosis level of K1 increased and its proliferation,migration and invasion ability decreased.2 FoxM1 may be another breakthrough point for the treatment of thyroid cancer.3 On the basis of simple chemotherapy,combined use of siRNA-FoxM1,or can improve the prognosis of patients with FoxM1 overexpression.