| Part one Experimental study on Microleakage of Embrace WetbondObjective: The aim of this study is to observe the microleakage of Embrace Wetbond pit and fissure sealant with and without saliva contamination,compared to the traditional resin sealant and flowable composite,and to provide experimental guideline for the clinical application.Methods:1.There were ninety six extracted healthy premolars collected from The Department of Oral &Maxillofacial Surgery,Dental Hospital of Hebei Medical University,which were cleaned and preserved in the 1% chloramine at 4℃.Teeth were divided randomly into 8 groups(n=12),and were performed pit and fissure sealant as following groups:A1 cleaning teeth +acid etching + rinsing and drying + 3M Concise.B1 cleaning teeth +acid etching + rinsing and blowing slightly +Embrace Wetbond.C1 cleaning teeth + drying + Constic.D1 cleaning teeth +acid etching + rinsing and drying + Beautifil Flow PlusF10.A2 cleaning teeth +acid etching + rinsing and drying +saliva contamination+ drying+ 3M Concise.B2 cleaning teeth +acid etching + rinsing and blowing slightly + saliva contamination+ blowing slightly +Embrace Wetbond.C2 cleaning teeth + drying + saliva contamination+ drying + Constic.D2 cleaning teeth +acid etching + rinsing and drying + saliva contamination+ drying+ Beautifil Flow PlusF10.2.All teeth with pit and fissure sealants were immersed in artificial saliva at the temperature of 37 ℃ for 30 days.3.Thennocycling test: the samples were placed in 0-5 ℃,55-60 ℃ water bath,then each bath stay 30 s for a cycle and repeated 500 times.4.After thennocycling test,each group randomly selects 6 specimens observed their gaps between fissure sealant and the tooth with Scanning Electron Microscope(SEM);the other 6 teeth of each group with methylene blue were observed marginal microleakage with Stereo Microscope.5.Recorded and graded microleakage of each sample,gap widths between fissure sealant and the teeth by the Scanning Electron Microscope,then make statistical analysis.Results:1 The results of Stereo Microscope:1.1 In the groups without saliva contamination,the microleakage of the four groups arranged in order of large to small: C1,B1,A1 and D1,the microleakage among the four groups was significant difference(χ2 = 102.621,P<0.05).The comparison between the four groups showed that C1 was the largest,and the differences between C1 and other three groups were statistically significant,respectively(P<0.05);B1>A1,B1>D1 and there was no significant difference among them,respectively(P>0.05);there was no significant difference between A1 and D1(P>0.05).1.2 In saliva contamination groups,the microleakage of the four groups arranged in order of large to small: C2,B2,A2 and D2,the microleakage among the four groups was significant difference(χ2 =80.331,P<0.05).The comparison between the four groups showed that C2 was the largest,and the differences between C2 and other three groups were statistically significant,respectively(P<0.05);B2>A2,B2>D2 and there was no significant difference among them,respectively(P>0.05);There was no significant difference between A2 and D2(P>0.05).1.3 The same material control with saliva contamination or not results: There differences between A1 and A2,D1 and D2 were statistically significant,respectively(P<0.05);There was no significant difference between B1 and B2(P>0.05);C1 and C2 microleakage were the largest,and there was nosignificant difference between them(P>0.05).2 The results of SEM:2.1 In the groups without saliva contamination,the gap widths between fissure sealant and enamel surface were groupC1>B1>A1>D1,there were significant differences in the gap widths among the four groups(χ2 = 19.641,P <0.05);The gap width of C1 was the largest,and the differences with A1,B1 and D1 were statistically significant,respectively(P<0.05);the gap widths in the B1 was the second,and the differences with A1 and D1 were statistically significant,respectively(P<0.05);A1 and D1 gap widths were smallest,there was no significant difference between them(P> 0.05).2.2 In saliva contamination groups,the gap widths between pit and fissure sealant and enamel surface were group C2>B2>A2>D2,there were significant differences in the gap widths among the four groups(χ2 = 19.727,P <0.05);The gap widths of C2 was the largest,and the differences with A2,B2 and D2 were statistically significant,respectively(P<0.05);the gap widths in the B2 group was the second,and the differences with A2 and D2 were statistically significant,respectively(P<0.05);A2 and D2 gap widths were smallest,there was no significant difference between them(P> 0.05).2.3 The same material control with saliva contamination or not results: there were significant differences between A1 and A2,C1 and C2,D1 and D2,respectively(P <0.05);there was no significant difference between B1 and B2(P> 0.05).Part two Experimental study on Microleakage of BeautiSealant.Objective: The aim of this study is to observe the microleakage of BeautiSealant by stereomicroscope and SEM,meanwhile compared to that of traditional resin fissure sealant and flowable composite,and to provide guideline for BeautiSealant fissure sealant’s clinical application.Methods:1.The thirty six in total extracted healthy premolars were collected from The Department of Oral &Maxillofacial Surgery,Dental Hospital of Hebei Medical University,cleaned and preserved in the 1% chloramine at 4℃.Teethwere divided randomly into 3 groups(n=12),and were performed pit and fissure sealant as following groups:A cleaning teeth +acid etching + rinsing and drying + 3M Concise.B cleaning teeth + BeautiSealant primer+drying+BeautiSealant.C cleaning teeth +acid etching + rinsing and drying + Beautifil Flow PlusF10.2.All specimens with pit and fissure sealants were immersed in artificial saliva at constant temperature of 37 ℃ for 30 days.3.Thennocycling test: the samples were placed in 0-5 ℃,55-60 ℃ water bath,then each bath stay 30 s for a cycle and repeated 500 times.4.After thennocycling test,each group randomly selects 6 specimens observed their gap width between fissure sealant and teeth with Scanning Electron Microscope(SEM);the other 6 teeth of each group with methylene blue were observed marginal microleakage with Stereo Microscope.5.Recorded and graded microleakage of each sample,gap the width between the fissure sealant and teeth by the Scanning Electron Microscope,then make statistical analysis.Results:1.The results of Stereo Microscope:There was significant difference in the microleakage among the three groups(χ2 =11.399,P<0.05);The microleakage of A and C were smaller,which was no significant difference between them(P>0.05);the microleakage of B was larger,and there were significant differences with other two groups,respectively(P<0.05).2.The results of SEM:There was significant difference in the gap width among the three groups(χ2 = 11.676,P<0.05);The gap widths of A and C were smaller,there was no significant difference between them(P>0.05).The gap widths of B was larger,there were significant differences with other two groups,respectively(P<0.05).Conclusion:1.The results of Stereo Microscope: whatever there was salivacontamination or not,the microleakage of Constic was the largest;and there was no significant difference in that of among Embrace Wetbond,3M Concise and Beautifil Flow PlusF10(P>0.05)2.The results of SEM: whatever there was saliva contamination or not,the gap widths of Constic was the largest;Embrace Wetbond was the second,and 3M Concise and Beautifil Flow PlusF10 were the smallest.3.Saliva contamination had no influence on the microleakage or gap width of Embrace Wetbond,however it had a significant influence on the microleakage and/or gap width of 3M Concise,Beautifil Flow Plus F10 and Constic.4.The microleakage of BeautiSealant was significant larger than that of3 M Concise and Beautifil Flow PlusF10.5.The gap width between pit and fissure sealant and enamel of BeautiSealant was significant larger than that of 3M Concise and Beautifil Flow PlusF10. |
PDF Full Text Request