Bioinformatics Analysis Of MicroRNA-183 In Colorectal Cancer

Background: Colorectal cancer(CRC)is a common malignant neoplasm.MicroRNAs are considered to play an important role in tumorigenesis and tumor progression.It has been reported that miR-183 is disordered in various tumors,however the role of miR-183 in CRC is still not fully elucidated.Objective: To compare the expression levels of miR-183 in CRC by multiple public databases,and explore their potential biological functions by bioinformatics analysis.Methods: We identified the differential expression of miRNAs in CRC by analyzing the public database TCGA,and confirmed the expression of miR-183 in GEO.Then we predicted the target gene aiming to investigate the biological function of miR-183 through Gene Ontology(GO)analysis and Kyoto Encyclopedia of Gene and Genome(KEGG)pathway analysis.Furthermore,Search Tool for the Retrieval of Interacting Genes/Proteins(STRING)was utilized to visualize the protein-protein interaction(PPI),for screening out the key genes that may be regulated by mi R-183.Results: There were 107 and 110 up-regulated,473 and 319 down-regulated miRNAs individually in colonic adenocarcinoma and rectal adenocarcinoma in TCGA.The higher expression of mi R-183 in CRC also confirmed in GEO.Further predictive research showed PIK3 CA,PRKACB and CCNB1 may regulated by high expressed miR-183 in CRC.Conclusion: The expression of miR-183 was significantly increased in CRC tissues.It might regulate the level of PIK3 CA,PRKACB and CCNB1 in the development of CRC.Although our approach may enable genetic changes to be identified more efficiently,experimental verification is essential to confirm the present results.