The Mechanism Of Regulation Of High-glucose Induced Oxidative Stress In Liver By Baicalein

Diabetes has become one of the diseases with the highest morbidity and mortality in the world,and the complications of diabetes also seriously threatened the health and safety of patients.Commonly used drugs such as acarbose,metformin,etc.,although it can reduce blood glucose,but often accompanied by the occurrence of adverse reactions.Baicalein is a flavonoid which has anti-inflammatory,antioxidant,and antibacterial activities.It has also been found to reduce blood glucose and improve diabetic complications.However,the mechanism of its therapeutic effect is still not clear.Therefore,this dissertation mainly studied the anti-oxidative and anti-apoptosis effects of baicalein on cells and tissues induced by high glucose,and analyzed its molecular mechanism of resisting oxidative stress and apoptosis from the perspective of signal pathway.The main research content includes the following aspects:A model of high glucose induced normal liver cell HL7702 cells was established.It was determined that 60.5 mM was the optimal glucose-induced concentration.The MTT assay was used to examine the effects of different concentrations of baicalein and high glucose on cell survival.The survival rate of high glucose treated cells was significantly lower than that of normal cells,and cell viability was improved after baicalin treatment.The effects of baicalein on oxidative stress and apoptosis induced by high glucose were investigated.The changes of SOD,CAT,GSH and MDA content in the cells were detected by spectrophotometry.The ROS content was detected by DCFH-DA probe,and the change of apoptosis level was detected by Annexin V/PI double staining technique.After baicalein treated cells,intracellular SOD,CAT,GSH levels increased,and MDA levels decreased significantly.ROS levels decreased and early apoptosis rates decreased significantly.The anti-oxidation parameters of liver tissues of diabetic mice were similar to those in cells.The molecular mechanism of baicalein against high glucose-induced oxidative stress and apoptosis was studied.Throurh analysis of gene expression profiles of liver tissues of diabetic mice administered with baicalein,PERK/Nrf2/CHOP pathway in the protein processing in endoplasmic reticulum was significantly regulated.At the cellular level,qRT-PCR and Western blot analyses showed that baicalein inhibited the expression of PERK and CHOP proteins and mRNA in HL7702 cells induced by high glucose,and upregulated the expression of Nrf2 and HO-1 proteins and mRNA.At the level of animal tissues,the expression pattern of each protein was consistent with the cellular results,and it could upregulate the expression of anti-apoptotic protein Bcl-2 and downregulate the expression of pro-apoptotic protein Bax.