Expression Of Serum Long Non-coding RNA NEAT1 In Lung Adenocarcinoma And Its Clinical Significance

Objective: To detect the expression of long non-coding RNA(lncRNA)nuclear paraspeckle assembly transcript 1(NEAT1)in serum of lung adenocarcinoma patients,and to analyze the relationships between the expression level of NEAT1 and the clinical pathological characteristics of lung adenocarcinoma patients.Furthermore,by investigating the serum level and clinical significance of lncRNA NEAT1 in lung adenocarcinoma,aimed to provide a new theoretical basis for clinical application of NEAT1 in the serological diagnosis biomarker and therapy for patients with lung adenocarcinoma.Methods:1 Sterile peripheral venous blood specimens in an empty stomach were collected from 42 cases of lung adenocarcinoma patients whose pathological diagnosis was confirmed in the Fourth Hospital of Hebei Medical University between June and December,2017,and 40 cases of normal healthy people as control group at the same time.Each specimen centrifuged for 10 minutes at 1200 g after letting stand at room temperature for 30 minutes.Then the upper light yellow serum transfered centrifuged at the condition of 4°C,12000 g for 10 minutes.250?l serum was selected and added 750?l TRIzol?LS reagent respectively,storaged in-80°C ultra-low temperature refrigerator for later use after enough mixed them together.2 According to TRIzol?LS reagent specifications,total RNAs from serum specimens of patients with lung adenocarcinoma and healthy controls were extracted strictly respectively.The first chain cDNA was synthesized using a reverse transcription kit after RNA purity identification and integrity detection qualified.When the reaction terminated,the cDNA were refrigerated in the condition of-20°C for reservation.3 Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the serum expression of NEAT1 in 42 patients with lung adenocarcinoma and 40 healthy controls.The relationships between the serum expression level of NEAT1 and clinical pathological characteristics were analyzed.Results:1.The expression of NEAT1 in serum of lung adenocarcinoma patients and healthy controls.Compared with 40 healthy controls,the serum expression level of NEAT1 in lung adenocarcinoma patients was upregulated,the difference between the two groups was statistically significant(t=3.869,P<0.05).2.The relationships between the expression of NEAT1 in serum and clinical pathological characteristics of lung adenocarcinoma patients.After Statistical analysis,the expression of NEAT1 in lung adenocarcinoma patients with tumor sized >3cm were significantly higher than that of tumor size ?3cm(t=9.299,P<0.01).Compared to those no lymph node metastasis patients,the expression of NEAT1 in serum of patients with lymph node metastasis was significantly upregulated(t=7.686,P<0.01).In addition,compared to patiens of TNM(I+II)stage lung adenocarcinoma,the expression level of NEAT1 in serum with TNM(III+IV)stage was significantly upregulated(t=6.300,P<0.01).Therefore,the expression of NEAT1 in serum was correlated significantly with tumor size,lymph node metastasis and clinical TNM stage(P<0.01),while there was no significance among age,gender,smoking,and tumor differentiation(P>0.05).Conclusion: The expression of NEAT1 in serum of lung adenocarcinoma patients was upregulated significantly than that of healthy controls,and was correlated significantly with tumor size,lymph node metastasis and clinical TNM stage,while not with age,gender,smoking,and tumor differentiation,which indicated that NEAT1 might play crucial oncogenic role in the progression of lung adenocarcinoma,and might become a novel potential serological biomarker for the diagnosis and therapy of lung adenocarcinoma.