Effect Of SiRNA Mediated FoxM1 Silencing On Proliferation And Migration Of Nasopharyngeal Carcinoma 5-8F Cells Via Wnt/β-catenin Signaling Pathway

Objective:To investigate the effect of siRNA mediated FoxMl silencing on malignant behaviors of nasopharyngeal carcinoma cell line 5-8F via Wnt/β-catenin signaling pathway.Methods:The FoxM1-siRNA by chemical synthesis was transfected into 5-8F cells,and the blank and negative control cells were set up.Real-time PCR(RT-PCR)and Western blotting were used to detect the mRNA and protein levels of FoxMl respectively.MTT assay,flow cytometry and Transwell chamber test were used to determine the effect of FoxM1 on proliferation,apoptosis,cell cycle distribution,invasion and migration of 5-8F cells.The protein expression levels of β-catenin,C-Myc,Cyclin D1,MMP-2 and MMP-9 were detected by Western blotting.Results:The mRNA and protein levels of FoxMl were decreased obviously after FoxMl-siRNA transfection in 5-8F cells(P<0.05).When compared with the blank and negative control cells,the silence also inhibited the proliferation(P<0.05),but enhanced apoptotic rate[(22.68±0.67)%vs(10.94±1.52)%and(11.74±1.36)%,P<0.05],arrested the cell cycle at GO/G1 phase,and decreased the number of invaded cells(100.00±10.97 vs 233.70±12.41 and 246.00±6.66)and migratory cells(33.33±2.40 vs 60.67±1.45 and 60.33±3.18)(P<0.05).Furthermore,Western blotting demonstrated that the nuclear protein level of P-catenin and the total protein levels of C-Myc,Cyclin D1,MMP-2 and MMP-9 were decreased after FoxM1-siRNA transfection(P<0.05).Conclusion:siRNA mediated FoxMl silencing increases the apoptotic rate,inhibits the proliferation,reduces the migration,and arrests cell cycle at GO/G1 phase in nasopharyngeal carcinoma 5-8F cells.Its mechanism may be through suppressing the expression of β-catenin,key protein of Wnt signaling pathway,and thus inhibiting the activation of the pathway.