A Study Of The Effect Of MiR-34a-5p On HaCaT Cell Proliferation And Notch1?Foxp1 Gene Expression

Objective:To estimate the effects of mi R-34a-5p on cultured HaCaT human keratinocytes and its target gene Notch1?Foxp1 m RNA expression.To study the possible mechanism of mi RNA in the pathogenesis of psoriasis.Methods:HaCaT cells culture and optimized transfection concentration were divided into 5 groups:micro RNA-34a-5p mimic group(mimic),negative control group(NC),mi R-34a-5p inhibitor group(inhibitor),inhibitor negative control group(inhibitor NC),blank control group.MTT assay was used to detect the proliferation of the transfected cells.The total RNA of the transfected HaCaT cells was extracted,and the expression of mi RNA-34a-5p,notch1 m RNA and foxp1 m RNA was detected by reverse transcription RT-q PCR.Results:After 48 hours of transfection,the cell viability of mimic group,inhibitor group,NC group and inhibitor NC group was 39.7%,15.7%,21.3%,and 20.7%,respectively.Compared with blank control group,the difference was statistically significant(P<0.05).Real-time fluorescent quantitative detection of Notch1 and Foxp1 expression in the mimic group,inhibitor group,NC group,and inhibitor NC group after transfection of HaCaT cells: The expression levels of notch1 m RNA in the mimic group and the inhibitor group were 2.78�1.30 and 0.37�0.46,respectively.Compared with the blank control group,the difference was statistically significant(P<0.05).The expression of Foxp1 m RNA in the mimic group and inhibitor group was 1.43�0.29 and 0.41�0.18,respectively.Compared with the blank control group,the difference was statistically significant(P<0.05).Conclusion:MiR-34a-5p can promote the proliferation of HaCaT cells in vitro,and its mechanism may be related to the up-regulation of Notch1 m RNA and Foxp1 m RNA in mi RNA-34a-5p.MiR-34a-5p may be involved in the pathogenesis of psoriasis by regulating downstream target genes Notch1 and Foxp1.