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Research On Contrast-enhanced Ultrasound To Evaluate Chronic Alcoholic Testicular Spermatogenic Function Damage Caused By Microvascular Changes

Posted on:2019-02-02Degree:MasterType:Thesis
Country:ChinaCandidate:L C HongFull Text:PDF
GTID:2404330569481369Subject:Imaging and nuclear medicine
Abstract/Summary:PDF Full Text Request
Objective To explore the application value of contrast-enhanced ultrasound technology in evaluating the chronic alcoholic testicular spermatogenic function damage caused by microvascular changes.Methods A total of 36 healthy adult male New Zealand rabbits were randomly divided into 6 groups including control groups(Group S1,S2,S3),alcohol treatment groups(Group T1,T2,T3)with 6 in each group.Adaptive feeding after one week,all the groups were given with nomal diet every day.Meanwhile,the control groups were given by gavage with normal saline 5 ml/kg while the alcohol treatment groups were given by same way with 60% alcohol 5 ml/kg the other day.S1,T1 group were fed 30 days,S2,T2 group were raised 45 days and S3,T3 group were fed 60 days.Rabbits in each group were performed on contrast-enhanced ultrasound(CEUS)after the successful build of each model,followed by analyzing CEUS parameters including PI,TP,Slope,MTT,DT/2 and AUC with the quantitative analysis technologies and comparing both results of evaluation.Meanwhile,rabbits in each group were performed on the heart puncture after CEUS to obtain 5ml blood for the laboratory testing of the plasma level of ET-1 and NO.HE staining,Johnsen's scoring and MDA detection were performed on the test of the same side after blood sample collection for observation of the pathological changes and comparison of changes about MDA level of each group.Results Expression of CEUS: There was no significant difference in parameters of CEUS among the control groups(P>0.05).There was no significant difference in parameters of CEUS between group S1 and T1(P>0.05).Compared with group S2,the peak intensity of group T2 decreased,and the PI decreased(P < 0.05),and there was no significant difference in TP,Slope,MTT,DT/2 and AUC(P>0.05).Compared with group S3,the PI and AUC of group T2 decreased obviously(P < 0.01),TP,MTT and DT/2 reduced(P<0.05),and there was no significant difference in Slope(P>0.05).Compared with group T1,the PI and AUC of group T2 decreased(P < 0.05),Slope decreased(P<0.05),MTT reduced(P<0.05)and there was no significant difference in TP and DT/2(P>0.05).Compared with group T2,the PI and AUC of group T3 decreased(P < 0.05),TP,MTT and DT/2 reduced(P<0.05),and there was no significant difference in PI(P>0.05).Detection of plasma: There was no significant difference in plasma level of ET-1 and NO among the control groups(P>0.05).Compared with group S1,plasma level of ET-1 and NO of group T1 increased(P < 0.05).Compared with group S2,plasma level of ET-1 and NO of group T2 increased obviously(P < 0.01).Compared with group S3,plasma level of ET-1 and NO of group T3 increased obviously(P < 0.01).Compared with group T1,plasma level of ET-1 and NO of group T2 increased(P < 0.05).Compared with group T2,plasma level of ET-1 and NO of group T3 increased obviously(P < 0.01).Detection of pathological tissue: The S group(S1,S2,S3)testicular tissue HE staining in the microscopic showed that in seminiferous tubules the sperm cells at all levels which mainly consists of spermatogonium and spermatocyte ordered clearly.The cellular structure was clear,and a large number of spermatid and small amounts of sperm rased inside the lumen.The HE staining of group T1 was similar to group S and a small number of exfoliated necrotic cells could be seen in some seminiferous tubules.The HE staining of group T2 in the microscopic showed that in seminiferous tubules the sperm cells at all levels which mainly consists of spermatogonium and spermatocyte were less clear than group S and T1.And a few spermatid and sperm rased inside the lumen,a small number of exfoliated necrotic cells could be seen in some seminiferous tubules.The HE staining of group T3 showed that in dilated seminiferous tubule the sperm cells at all levels which mainly consists of spermatogonium and spermatocyte had unclear boundary and a disordered arrangement.The cellular and nucleus structure was swelled and fuzzy,and the chromatin was not uniform.The number of sperm was less.A small number of exfoliated necrotic cells could be seen in some seminiferous tubules.Johnsen's scoring:There was no significant difference in the score of Johnsen's criterion among the control groups(P>0.05).Compared with group S1,the score of group T1 had no significant change(P>0.05).Compared with group S2,the score of group T2 decreased(P < 0.05).Compared with group S3,the score of group T3 decreased obviously(P < 0.01).Compared with group T1,the score of group T2 had no significant change(P>0.05).Compared with group T2,the score of group T3 decreased obviously(P < 0.01).MDA detection:There was no significant difference in MDA level among the control groups(P>0.05).Compared with group S1,the MDA level of group T1 increased(P < 0.05).Compared with group S2,the MDA level of group T2 increased obviously(P < 0.01).Compared with group S3,the MDA level of group T3 increased obviously(P < 0.01).Compared with group T1,the MDA level of group T2 increased obviously(P < 0.01).Compared with group T2,the MDA level of group T3 increased obviously(P < 0.01).Conclusions 1.The damage effect of alcohol on testicular spermatogenesis is time dependent.2.Chronic high concentration of alcohol can result in changes in the hemodynamics of testicular microcirculation,which is mainly manifested by decreased capacity,and results in the corresponding testicular spermatogenesis impairment.3.The quantitative analysis technique of CEUS can be used to evaluate the chronic alcoholic hemodynamic changes of testicular microcirculation,which can be used to indirectly reflect the alcoholic impairment of the testicular spermatogenic function.
Keywords/Search Tags:contrast-enhanced ultrasound, alcoholic injury, testicular microvascular, endothelial cells
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