Study On The Inhibitory Effect And Mechanism Of Psoralen On Prostate Cancer Cell PC3

Background:Prostate cancer is the most common tumor of the urinary system.In recent years,the morbidity of prostate cancer in China has been significantly increased.Because of the delitescence of prostate cancer,most of the patients have been found in the advanced stage,and the treatment is not effective,so it is urgent to find out a effective drug.Chinese medicine has unique viewpoint in the understanding and treatment of prostate cancer.Psoralen,the traditional Chinese medicine,has been found having the ability to inhibit the growth of various tumor cells,and is expected to be applied in the treatment of prostate cancer.In order to lay the foundation for elucidating the molecular mechanism of psoralen antitumor effect,we use gene chip technology to analyze the expression of lncRNA and mRNA in the PC3 cells before and after treating with psoralen,and then analyze the data with the help of GO database and KEGG database.Objective:Our goal is: 1.To determine the inhibitory effect of psoralen on the proliferation of PC3 cells.2.To research whether or not psoralen can causes changes in the cell cycle of PC3.3.Using gene chips to research whether there was differentially expressed genes between the psoralen treatment group and control group.4.Analyzing the differentially expressed genes to preliminarily identify the signal pathways and target molecules related to the function of psoralen.5.To verify the results of the gene chips and to determine the reliability of the results of the gene chips.Method:1.CCK8 was used to detect the effect of psoralen on the proliferation of PC3 cells.2.Flow cytometry was used to detect the changes of PC3 cell cycle.3.The changes of lncRNA and mRNA expression profiles of PC3 cells before and after psoralen treatment were analyzed by using gene chips.4.Using information of bioinformatics to analyze differentially expressed lncRNAs and mRNAs.5.Using RT-qPCR to verify the results of gene chips.Results:1.The proliferation of PC3 cells was significantly inhibited in varying degrees after treating with different concentrations of psoralen.2.The G0/G1 and G2/M cells of PC3 cells in psoralen treating group were more than those in the control group.3.The results of gene chips showed that there were 16426 LncRNAs were differential expressed(P < 0.05).The amount of lncRNAs that was differently expressed more than 2 times,and the p < 0.05 was 4985,of which 1716 were significantly increased,and 3269 were significantly reduced.The amount of mRNAs that was differently expressed more than 2 times,and the p < 0.05 was 4423,of which 1160 were significantly increased,and 3263 were significantly reduced.4.The molecular analysis of differentially expressed genes is as follows: GO analysis shows that a total of 663 GO items were related to the differentially expressed mRNAs in the experimental results.KEGG pathway enrichment analysis of differentially expressed mRNAs showed that there were 22 significantly related pathways.The GO database was used to predict the CIS target genes of differentially expressed lncRNAs.705 GO items were found to be related to the differentially expressed lncRNAs in the experimental results.181 GO related items were found by trans target gene prediction.The KEGG database was used to predict the CIS target pathways of differentially expressed lncRNAs.18 pathways were found related to the differentially expressed lncRNAs in the experimental results by trans cis pathway prediction,and 15 pathways were found by trans target pathway prediction.5.The results of RT-qPCR verification of gene chips are consistent with the results of gene chips.Conclusion:1.Psoralen can significantly inhibit the proliferation of PC3 cells,and this inhibitory effect is dependent on time and concentration.2.Psoralen can play an antitumor role by inducing the G0/G1 and G2/M phase block of PC3 cells.3.LncRNAs and mRNAs were differently expressed in the psoralen treating PC3 cell group and control group.4.The mechanism of the antitumor activity of psoralen is complex,it may be changing in multiple genes and multiple pathways.