The Establishment Of Human Skin Xenograft And Gene Introduction Model

Object:To study how to establish a model of human skin xenograft and gene introduction model Transplantation of human skin on immunodeficient mice would be an invaluable tool for investigating mechanismsinvolved in psoriasis,skin tumor,wound healing and skin hereditary disease.Methods : Firstly,theskin of newborn baby black mouse was rinsed with 10% disinfectant water,75% ethanol and sterile saline in turn,.Cut into 1–1.5cm2(transplantation bed)pieces by eye scissors.Sliced tissues without faults were spread with the epidermis facing upwards.Nude mice were anesthetized by chloral hydrate.The mouse skin was moved on transplantation bed,andbinded by bandages which were opened in 14 days.Secondly,theskin of human was rinsed with 10% disinfectant water,75% ethanol and sterile saline in turn,.Cut into 1–1.5cm2(transplantation bed)pieces by eye scissors.Sliced tissues without faults were spread with the epidermis facing upwards.NOD-scid mice were anesthetized by chloral hydrate.The mouse skin was moved on transplantation bed,andbinded by bandages which were opened in 14 days.Thirdly,if the human skin was in good condition,about in the third week,itwould be infected virus which included GFP by intradermal injection.Results:In two weeks,first,The skin of the newborn black mouse transplanted to the waist of the nude mice survived better,the hair was dense,and the pigment was seen in some areas.Second,The prepuce of the man transplanted to the nude mouse was poor,dark red,visible contraction and abscission.Third,The prepuce transplanted to the nude mice can be seen at the waist of nod-scid mice,with a normal skin tissue structure,and a report protein(GFP)detected in the transplanted foreskin.Conclusions:The prepuce transplanted to the nod-scid mice retained normal tissue structure,andinfected with a virus that reports protein,.This provides a good model for skin tumor,wound healing,skin genetic disease and so on.The research model is widely used in basic research,drug discovery,development and clinical medicine of human skin diseases.