The Expression Of SOX10 Protein,GATA3 And CK19 In Extramammary Paget's Disease With Its Biological Significance

Object:Extramammary Paget's disease(EMPD)is considered an uncommon carcinoma in situ that can infiltrate the dermis and even metastasize to local lymph nodes and distant sites.which although it occurs most commonly in the anogenital region,can arises in any area of skin or mucosa.Primary EMPD is a form of intraepithelial adenocarcinoma of uncertain histogenesis.Currently the following theories are supported :Cells in the so-called mammary-like glands(MLG)of the anogenital region,or in the skin adherence gland.EMPD is mainly observed in skin regions that are rich in apocrine and apoeccrine glands and thus most commonly affects the external anogenital region as well as the axilla.Based on the above reasons,most experts and scholars believe to it.In addition,for Toker cells of the epidermis,Marlucci and others indicate that Toker cells are most likely the precursor of EMPD.pluripotent stem cells and infiltrating urothelial carcinomas with involvement of the epidermis have beendiscussed as histogenetic origins of EMPD.Hence,The objective of this study is to explore cell origins of EMPD by immunohistochemical markers SOX10?GATA3 and CK19.Also,to further improve the immunohistochemical markers of EMPD and illustrate the biological significance of these markers in EMPD.Methods:1.Collecting 60 cases of extramammary Paget's disease which were diagnosed by histopathology in the First Affiliated Hospital of Fujian Medical University,between 2005 to 2017.2.The paraffin specimens were sliced,baked and stained with immunohistochemical method.To observe the positive expression and expression pattern about immunohistochemical markers of SOX10,GATA3 and CK19 in extramammary Paget's disease.3.The statistical software SPSS20.0 was used to analyze the data obtained,so as to obtain the expression of the above three immunohistochemical markers in extramammary Paget's disease.Results:1.The expression of immunohistochemical marker SOX10 in extramammary Paget's disease: 60 cases of extramammary Paget's disease,positive expression in 0 cases,negative expression of 60 cases.But it can be expressed in the small sweat glands.2.The expression of immunohistochemical marker GATA3 in extramammary Paget's disease: in 60 cases of extramammary Paget disease,42 cases were strongly positive,12 cases were moderately positive,and 6 cases were weakly positive.But both were negative in the small sweat glands.3.The expression of immunohistochemical marker CK19 in extramammary Paget's disease: all cases of diffuse strong positive expression.Strong positive expression was found in the small sweat glands and in the sweat glands.Conclusion:1.The expression of SOX10 in extramammary Paget's disease is negative,and the expression in the small sweat glands is positive,suggesting that the Paget cells do not originate in the small sweat glands.2.The expression of GATA3 was positive in Paget cells in extramammary Paget disease,and suggesting that extramammary Paget disease may be derived from the apocrine sweat gland.3.The expression of CK19 in Paget cells in extramammary Paget disease is strongly positive,and it is also strongly positive in apocrine glands,suggesting that Paget cells may originate from pluripotent stem cells.