| Tumor immunotherapy is a treatment that relieves or cures cancer by regulating the patient’s own immune system.Compared with traditional treatment methods,immunotherapy has the advantages of small toxic and side effects,improved patient survival and quality of life.Therefore,tumor immunotherapy has attracted more and more attention and research in recent decades.Vaccine treatment is one of the commonly used methods in immunotherapy.It has attracted attention due to its advantages of component determination,rapid activation of the immune system,small side effects,and good prognosis.Superantigen is a small molecule protein derived from microorganisms,which can nonspecifically stimulates the activation and proliferation of most T lymphocytes or B lymphocytes at low concentrations.Therefore,using it as an immunization vaccine can quickly activate a large number of lymphocytes in vivo to exert an immune effect.This experiment is to study the effect of superantigen and immunoadjuvant combination as a vaccine on the transplantation of reconstructed mouse breast tumors.First,4T1 cells were injected into the mouse mammary fat pad to construct a mouse breast tumor model.Then the superantigens were treated with different immune adjuvants to treat the mice before or after transplantation.The tumor volume changes were measured and then used.The proportion of T lymphocytes,B lymphocytes,and MDSC in total lymphocytes in peripheral blood,spleen,lung,and bone marrow of mice was measured by flow cytometry.Test results are as follows:(1)The use of Freund’s adjuvant in combination with superantigen to immunize mice can effectively inhibit the growth of transplanted tumors;(2)The percentage of B lymphocytes in the spleen was significantly increased in the immunized mice compared to the transplanted control group and was closest to the healthy control group:(30.90 ± 0.90)% in the experimental group,(9.75 ± 0.75)%(n=5,P<0.01)in the transplantation control group,(36.25 ± 0.75)% in the healthy control group;(3)The percentage of MDSC in the lungs of mice immunized with immunized mice was significantly lower than that in the transplanted control group:(6.92±0.58)% in the experimental group,(58.16±0.84)%(n=5,P<0.01)in the transplant control group,and(2.60 ±0.6)% in the healthy control group.The conclusion reached through this experiment is: The superantigen vaccine prepared in our laboratory has a significant effect on the suppression of transplanted tumors in mice,suggesting that superantigen vaccines may achieve anti-tumor effects through humoral immunity or by regulating the ratio of inhibitory cells to lymphocytes in vivo. |
