Changes And Clinical Significance Of Peripheral Blood Antigen,Lymphocyte Subsets And HBV-DNA In Patients With Chronic Hepatitis B Infection Under Different Pathological Conditions

Objective:Study the changes of hepatitis B surface antigen(HBsAg),e-antigen(HBEAG),core antibody(Hbcab),T lymphocyte subsets and HBV-DNA load in peripheral blood of patients with chronic hepatitis B virus infection(CHB)with different inflammation and fibrosis state and their correlation,understand the changes of cellular immune function and the relationship between the corresponding serological indices and the progression of the disease in different pathological conditions of the liver.Methods:A total of 96 patients with chronic HBV infection patients as the research object,the patients received liver biopsy,and the pathological results of inflammatory activity and fibrosis for classification,and the chemiluminescence method for the determination of peripheral blood of patients with HBsAg,HBeAg,HBcAb content;and the use of flow cytometry determination of peripheral blood CD4~+T,CD8~+T and the level of Treg cells;the content of serum HBV-DNA of Roche DNA detection,statistical analysis was made between the different groups.Results:1.Serum HBsAg and HBeAg levels were significantly lower in CHB patients with inflammatory activity?G2 than those in patients with inflammatory activity<G2(P<0.05).HBcAb levels were significantly higher in CHB patients with inflammatory activity<G2(P<0.05);Serum levels of HBsAg and HBeAg in CHB patients with fibrosis grade?S2 were significantly lower than those in CHB patients whose fibrosis stage was<S2(P<0.05),and HBcAb levels were significantly higher than those of<S2 CHB.Patients(P<0.05).2.There was no significant difference in liver inflammation between HBeAg-negative patients and positive patients(P>0.05),while the degree of fibrosis was significantly higher than that of positive patients(P<0.05).The higher the HBeAg content,the degree of liver inflammation and fibrosis Decline(P<0.05).3.There was no significant correlation between serum HBsAg quantification and lymphocyte subsets(P>0.05),but there was a significant positive correlation between serum HBeAg quantification and Treg cell content(P<0.05),and there was a significant negative correlation between serum HBcAb quantification and CD8~+T cells(P>0.05).The patients were divided into negative and positive groups according to HBeAg.Statistical analysis showed that the number of treg cells in the HBeAg positive group was significantly higher than that in the negative group,and the difference was statistically significant.(P<0.05).4.The percentage of CD4~+T cells in the?G2 group was significantly higher than that in the<G2 subgroup.There was a significant difference between the two groups(P<0.05).The percentage of CD8~+T cells and the percentage of treg cells decreased with the increase in the degree of inflammation.There was a significant difference between the two groups(P<0.05),spearman correlation analysis showed that CD4~+T cells were positively correlated with liver tissue severity(r=0.417,p<0.05),CD8+T cells,and treg cells.There was a significant negative correlation in liver severity(r=-0.347,r=-0.337;P<0.05).There was no significant difference in peripheral blood lymphocyte subsets between patients with different fibrosis stages(P>0.05).After spearman correlation analysis,there was no difference between the degree of liver fibrosis and peripheral blood lymphocyte subsets.Obvious correlation.5.The percentage of CD4~+T cells in G2 group was significantly higher than that in group<G2,the difference between the two groups was significant(P<0.05),CD8~+T cell percentage and the percentage of Treg cells decreased with aggravation of inflammation and the comparison between the two groups had significant difference(P<0.05),Spearman correlation analysis showed that CD4~+T cells in liver tissues and the severity of positive related(r=0.417,p<0.05),CD8~+T cells,Treg cells and the severity of liver was negatively correlated(r=-0.347,r=-0.337;P<0.05).There was no significant difference in peripheral blood lymphocyte subsets between different stages of fibrosis(P>0.05),and there was no significant correlation between liver fibrosis and peripheral blood lymphocyte subsets after Spearman correlation analysis.6.There was no significant correlation between peripheral blood treg cell levels and Serum HBV-DNA levels(r=0.13,P>0.05).Conclusion:T lymphocyte subsets imbalance and cellular immune dysfunction exists in patients with CHB,hepatitis B antigen in different quantitative liver inflammation and fibrosis of CHB in peripheral blood of patients with differences in patients with inflammation and fibrosis of the heavier to reduce HBsAg and HBeAg content,increased HBcAb level,and the quantitative detection of antigen of lymphocyte subsets and levels of serum HBV-DNA can reflect the pathological state in a certain extent.