Construction And Mechanism Study Of Biological Protein Active Molecle And Drug Detection System

The study of biomolecules has made people understand the pathogenesis of some diseases at the gene level,and designed molecular probes to detect the content of internal molecules quantitatively,which provides a theoretical basis for the occurrence of various diseases.At the same time,it provides a certain theory for the use of dose in clinical medicine.Guide,and a lot of scientific basis.With the continuous development of drug detection and the emergence of nanoprobe detection technology,it not only provides a certain theoretical basis for the use of drug dose,but also plays an important role in human life.In this paper,the fluorescence properties of nitrogen doped carbon quantum dots are used to detect heparin in the form of protamine,respectively,as nanoprobe complexes and directly as fluorescence sensors.The main contents of this paper are as follows:?1?The citric acid was placed in monoethanolamine and the nitrogen doped fluorescent carbon dots were rapidly formed and synthesized by simple heating.Thenitrogen doped carbon quantum dots produced strong fluorescence emission at 458nm at 370 nm,and the maximum absorption wavelength was 365.085nm.Deoxyribonucleotides can enhance the fluorescence of the nitrogen doped carbon quantumdots and have a linear correlation.Therefore,the nitrogen doped carbon dots?NCDs?and deoxyribonucleotides hybridized nanocomposites?nanoprobes?were prepared.Ithasbeen successfully used for the detection of protamine.The principle is that thefluorescence of nitrogen doped carbon quantum dots is enhanced by the combination ofDNA and nitrogen doped carbon quantum dots through electrostatic interaction.With the addition of protamine,the competition of protamine is combined with DNA,thus the fluorescence of the system is restored.Based on this,a method for quantitative detection of protamine based on the properties of NCDs fluorescein is established.Under the best experimental conditions,the method is simple,selective,andso onThe linear detection range of the analysis method is 1-10?g.mL^-1,and the detection limit is up to 0.61?g.mL^-1.?2?The use of top-down liquid nitrogen doped carbon quantum dots were prepared by heating?NCDs?,heparin can enhance the fluorescence of carbon quantum dots and with linear relationship.Based on this phenomenon,an analytical method for the detection of heparin with a nitrogen doped carbon quantum dots as a fluorescence sensor is designed.The detection method takes advantage of the strong electrostatic interaction between NCDs and heparin to enhance the fluorescence of NCDs,thus detecting the content of heparin.The linear detection range of the method is 0.13².63 U/mL and the detection limit is 0.01 U/mL under the best experimental conditions.The recovery rate of the actual sample addition is between 93.88%¹01.23%.The nano molecular probe with photochemical stability,good water solubility,the characteristics of large surface area and so on,which greatly improves the detection performance of the traditional molecular probe.Carbon quantum dots?QDs?,based on carbon elements,have more biological safety and less interference to biological molecules than the previous fluorescent materials.Compared with metal quantum dots,carbon quantum dots can also give bright light under illumination,and have the characteristics of excitation light width and continuity,high stability and adjustable emission wavelength.When nitrogen atoms are added to carbon dots,because nitrogen atoms have lone pair electrons and enter into carbon skeleton,they will effectively regulate the properties of carbon dots themselves,such as surface or local,electronic characteristics and other chemical characteristics.In conclusion,in this paper,we use NCDs fluorescence properties to construct a nanoprobe for trace detection of biological molecules protamine and heparin.This method is simple and practical.It provides a theoretical basis for molecular biology and biomedical research,and provides some help for the prevention and treatment of some diseases at the early stage.