The Effect Of Low-frequency Stimulation In Medial Septum On Kainate-induced Epilepsy Model

Epilepsy is a common central nervous system disorder.Modern medicine and surgical procedures used for treatment of epilepsy have limitations in both therapeutic and clinical applications.Patients treated with seizure medication can easily develop refractory epilepsy.Deep brain stimulation(DBS)has become a hot topic in epileptic therapy,but there are currently no optimal target for DBS treatment and the mechanism for seizure prevention are unknown.Our previous study showed that the septo-hippocampal pathway was closely involved in the temporal lobe epilepsy(TLE).Hippocampal was the most common epileptic foci.Therefore,this study aims to explore whether DBS targeting MS is a potential target for the treatment of TLE.In the model of acute kainate-induced epilepsy,the electric stimulation of MS in different frequency(1 H2.5Hz?20Hz?100Hz)can inhibit generalized seizure and the effect of electrical stimulation in 5Hz was the most obvious.In the model of chronic kainate-induced epilepsy,low-frequency stimulation(LFS)of 5Hz in MS showed obvious inhibitory effect on focal seizure,but had no effect on generalized seizure.At the same time,LFS of 5Hz in MS can improve novel objection recognition task which can be impaired as a result of chronic epilepsy.Finally,spectrum analysis showed that LFS in MS can increase the theta rhythm in the hippocampus background electroencephalogram(EEG)rhythm in the model of acute kainate-induced epilepsy,but has no effect in the model of chronic kainate-induced epilepsy.Result suggests that there are different neuro-circuit pathways in various stages of TLE,and that the theta rhythm band of hippocampus is closely related to GS.Our research suggests that LFS of MS can alleviate acute and chronic kainate-induced epilepsy and improve cognitive function which hints that MS region of the brain may be a potential target for DBS or drug therapy for TLE.