All-trans Retinoic Acid Enhances The Effect Of Fra-1 Inhibition Cell Metabolism And Proliferation Of Cervical Cancer

Objective: To investigate the effect of retinoic acid enhancing Fra-1 on cell proliferation and energy metabolism in cervical cancer Hela and Caski cell lines,and to explore the possible mechanism of retinoic acid combined with Fra-1 influencing the biological effects of cervical cancer cells.Methods: 1.The cervical cancer Hela and Caski cell lines overexpressing the Fra-1 were constructed by lentivirus,screened with puromycin,and the results of overexpression were verified by Western blotting.2.Flow cytometry was used to analyze the levels of mitochondrial membrane potential and reactive oxygen species in cervical cells after over-expression of Fra-1 and retinoic acid..3.Detection of Fra-1 overexpression in combination with retinoic acid by the kit,lactic acid,adenosine triphosphate,and glucose consumption in cervical cancer cells.4.The effect of overexpression of Fra-1 combined with retinoic acid on cell proliferation was detected by CCK-8 and cell cloning experiments 5.Western blot was used to analyze the possible mechanism of the effect of overexpression of Fra-1 combined with retinoic acid on the biological effects of cervical cancer cells.Results:1.Western blot analysis confirmed that the lentivirus-transfected Fra-1 overexpressed cervical cancer cell line was successfully constructed.2.Overexpression of Fra-1 combined with retinoic acid caused a significant decrease in mitochondrial membrane potential and increase the concentration of intracellular reactive oxygen species in cervical cancer cells.3.Overexpression of Fra-1 can down-regulate the levels of cell lactate,adenosine triphosphate and glucose consumption,and retinoic acid can enhance this effect.4.The overexpression of Fra-1 combined with retinoic acid inhibited the proliferation of cervical cancer cells compared with the supernatant of Fra-1 and retinoic acid alone.5.Overexpression of Fra-1 combined with retinoic acid down-regulated the expression levels of MDM2,CDK-4 and LDHA,and upregulated the expression of p53 and p21 in cervical cancer cells.Conclusion: 1.All-trans retinoic acid combined with Fra-1 gene decreased mitochondrial membrane potential and increased intracellular reactive oxygen species level in cervical cancer cells.2.All-trans retinoic acid combined with Fra-1 gene may reduce cell lactate production,glucose consumption and ATP content by affecting MDM2/P53/LDHA.3.All-trans retinoic acid combined with Fra-1 gene may inhibit the proliferation of Hela and Caski cells through MDM2/p53/p21/CDK-4.